The stereoselectivity and yield in glycosylation reactions are paramount but unpredictable. We have developed a database of acceptor nucleophilic constants (Aka) to quantify the nucleophilicity of hydroxyl groups in glycosylation influenced by the steric, electronic and structural effects, providing a connection between experiments and computer algorithms. The subtle reactivity differences among the hydroxyl groups on various carbohydrate molecules can be defined by Aka, which is easily accessible by a simple and convenient automation system to assure high reproducibility and accuracy. A diverse range of glycosylation donors and acceptors with well‐defined reactivity and promoters were organized and processed by the designed software program “GlycoComputer” for prediction of glycosylation reactions without involving sophisticated computational processing. The importance of Aka was further verified by random forest algorithm, and the applicability was tested by the synthesis of a Lewis A skeleton to show that the stereoselectivity and yield can be accurately estimated.
The stereoselectivity and yield in glycosylation reactions are paramount but unpredictable. We have developed a database of acceptor nucleophilic constants (Aka) to quantify the nucleophilicity of hydroxyl groups in glycosylation influenced by the steric, electronic and structural effects, providing a connection between experiments and computer algorithms. The subtle reactivity differences among the hydroxyl groups on various carbohydrate molecules can be defined by Aka, which is easily accessible by a simple and convenient automation system to assure high reproducibility and accuracy. A diverse range of glycosylation donors and acceptors with well‐defined reactivity and promoters were organized and processed by the designed software program “GlycoComputer” for prediction of glycosylation reactions without involving sophisticated computational processing. The importance of Aka was further verified by random forest algorithm, and the applicability was tested by the synthesis of a Lewis A skeleton to show that the stereoselectivity and yield can be accurately estimated.
A real‐time digital signal processor for an earth rotation synthesis radio telescope is described. The processor accepts 4 MHz baseband signals, quantizes them to three levels, and performs delay equalization, cross correlation, and phase rotation. The inphase cross correlation of the input signals is calculated for 16 values of delay spaced by 62.5 ns. The quadrature correlator output is produced from the inphase output by numerical computation by using a band‐limited form of the Hilbert transform. The values of inphase and quadrature visibility at the exact value of delay are derived by interpolation. Errors arising in this signal processing method are investigated and shown to be small in the adopted design. A high‐speed, 16‐bit microcomputer is employed to allow many of the required operations to be performed in software. The processor produces four complex interferometer outputs simultaneously and is capable of producing ten such outputs. Interferometer visibility records of the radio source 3C66 are presented.
Optimizing glycosylation conditions for automated glycan assembly is highly challenging, demand wasteful use of precious building blocks and rely on nontrivial analyses. We developed a semi-quantitative method for automated optimization...
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