Milk’s qualitative and technological properties are greatly affected by genetic polymorphisms in the kappa-casein gene, and their polymorphisms may serve as informative markers of yield and composition. Thus, the objective of this study was to detect kappa-casein (kappa-CN) gene polymorphisms and their association with milk production traits in crossbred dairy cows. One hundred healthy crossbred (Friesian x Jenoubi) dairy animals between three and five years old were sampled for blood and milk during their first lactation. The genomic DNA was extracted from whole blood, and restriction fragment length polymorphism (RFLP-PCR) was used to determine the genotype of the kappa-CN gene. As a consequence of the restriction digestion of this fragment with Hind III, it showed three different restriction patterns: BB (453 base pairs uncut), AB (453, 206, and 225 base pairs), and AA (206 and 225 base pairs). Based on genetic diversity, the AB genotype was the most predominant (n = 67), with a frequency of 0.67. A variant genotype of the kappa-CN gene was associated with milk production traits in crossbred dairy cows. Animals with the AA variant produced a higher milk yield and a higher percentage of fat, casein, protein, and solids not fat (SNF) (P≤0.05) (1.397kg, 0.75%, 0.31%, 0.27%, and 0.68%, respectively) than those with the BB variant. A logistic regression analysis confirmed that the kappa-CN genotypes increase milk yield and casein content. Therefore, genetic variants of the kappa-CN gene could be used as genetic markers for improving milk production traits in dairy cattle.
Spirulinal powder supplementation is being studied to see if it can help slow the progression of aging in the heart. As a control group, twenty male rabbits were randomly and equally divided into four groups, each receiving 150 mg/kg BW of d-galactose S/C daily as Group II; 500 mg/kg BW of Spirulinal orally as Group III; and 150 mg/kg BW daily S/C administration of d-galactose with 500 mg/kg BW orally as Group IV for four weeks. Using the specific stain Masson's Trichrome, stained sections of the aorta from the D-gal group (GII) and the combination group (CII) exhibit histological changes in the tissue (GIV). Tissue slices stained with Masson's Trichrome stain reveal the location of collagen and muscle fiber accumulation, which appears as a red keratin and muscle fiber accumulation area. Our findings show that Spirulinal powder, a feed supplement, has a protective effect against heart aging.
Alpha lipoic acid has both hydrophilic and hydrophobic properties and is widely dispersed in cellular membranes and the cytoplasm. It is one of the most powerful antioxidants for cells. The current research looked at the possible therapeutic benefits of alpha lipoic acid) in a male rat model of D-galactose-induced brain aging. G1 normal as control group, G2 animals were given 200 mg/kg bw.IP. daily of D-gal group for 30 days, and G3 animals were given 100 mg/kg. G4 D-gal + alpha lipoic acid group200mg/kg bw.IP. daily with 100 mg/kg. IP every day for 30 days.The accumulation of Beta amyloid in the brain and changes in serum oxidant/antioxidant levels Increased levels of oxidative stress indicators our data demonstrate that the D-gal aging model causes an increase in brain Beta amyloid deposition, as well as a substantial rise in serum MDA, GSH, CAT, and SOD levels. To summarize, our research focused on the brain damage caused by D-gal, which can be mitigated by intubating 100 mg/kg B.W.IP. of ALA to counteract its negative effects.
Objective: To see how dose of Silymarin aqueous extracts affected prolactin hormone gene expression in pituitary glands and Androgen hormone in serum samples of female experimental rats throughout nursing. Methods: 24 adult healthy, Two sets of pregnant rats were created (12 each group) and treated as follows for ten days postnatal. G 1 were intubated orally distal water serving as control while, G 2 were intubated orally Silymarin (200 mg\kg\day). Results: The findings show that Silymarin had a strong influence on mRNA–PRL expression in pituitary glands, with a rise in Estrogen and a decline in Progesterone levels. Conclusion: The focus of this study mention a new evidence of the role of Silymarin on the prolactin gene expression for increasing the milk production and level of estrogen & progesterone hormones.
Objective: To see how dosage of Silymarin effect on some serum prolactin ,estrogen and progesterone and some oxidant\ antioxidant in serum samples of female experimental rats throughout nursing. Methods: Twenty-four adult healthy, pregnant rats were divided into two groups (12rats/group) and treated as follows for ten days. G 1 were intubated orally distal water serving as control while, G 2 were intubated orally Silymarin (200 mg\kg\day), the following criteria were measured serum prolactin, estrogen, progesterone hormones, GSH, MDA, SOD and CAT. Results: The findings show that Silymarin had a significant increase influence in serum prolactin, estrogen hormone, SOD, CAT, MDA and a significant decrease in Progesterone and GSH. Conclusion: The focus of this study mention a new evidence of the role of Silymarin on the increasing prolactin, estrogen & progesterone hormones hormone and effect on some level of oxidant\ antioxidant.
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