The study was performed on 31 samples of porcine muscle (m. longissimus dorsi) of different qualities, ranging from extremely pale, soft and exudative (PSE) to dark red in color, firm in structure and dry in appearance (DFD) in order to determine the water solubility of muscle proteins 24 hr post mortem. It was found that the water solubility of muscle proteins depends on the quality of the muscle, and is lowest in extremely PSE muscle. It was demonstrated, with the aid of electrophoresis on agar and acrylamide gels, that some sarcoplasmic proteins which undergo precipitation 30 min after adding a citrate-phosphate buffer (pH 4.6) at temperature of 20°C are extracted in smaller amounts from PSE muscle than from normal muscle. One of these fractions was benzidine positive.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.