Thiosulphate Metabolism in the Animal Organism

Skarzynski, B; Szczepkowski, T; M, Weber

doi:10.1038/184994a0

Cited by 25 publications

(7 citation statements)

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“…A preferential formation of sulphate from the inner atom of thiosulphate, shown in Table 1, has also been observed by some authors with autotrophic bacteria (Peck & Fisher, 1962;Trudinger, 1964;Kelly & Syrett, 1966) and in the whole animal (Skarzynski et al 1959). The delayed appearance of the labelled outer atom in the form of sulphate obviously depends on the intermediate steps of oxidation of bivalent sulphur.…”

Section: Discussionsupporting

confidence: 72%

“…Thiosulphate may be oxidized to sulphate in whole animals (Nyiri, 1923;Skarzynski, Szczepkowski & Weber, 1959) or in slices from rat liver (Pirie, 1934). The mechanism ofthis oxidation was not elucidated until observations by Sorbo (1964) showed that addition of some thiols greatly increased sulphate production from thiosulphate incubated with rat liver homogenate in an oxygen atmosphere.…”

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confidence: 99%

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[35S]thiosulphate oxidation by rat liver mitochondria in the presence of glutathione

Koj¹,

Frendo²,

Janik³

1967

Biochemical Journal

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1. Rat liver mitochondria incubated in oxygen with glutathione and [(35)S]-thiosulphate produced labelled sulphate. 2. Inner-labelled thiosulphate (S.(35)SO(3))(2-) was converted into [(35)S]sulphate more rapidly than outer-labelled thiosulphate ((35)S.SO(3))(2-). 3. Thiosulphate labelled in both sulphur atoms was formed during ((35)S.SO(3))(2-) oxidation; the outer sulphur atom before oxidation to sulphate was incorporated into the inner position. 4. A thiosulphate cycle in the metabolic pathway of sulphate formation in animal tissues is discussed.

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Section: Discussionsupporting

confidence: 72%

mentioning

confidence: 99%

[35S]thiosulphate oxidation by rat liver mitochondria in the presence of glutathione

Koj¹,

Frendo²,

Janik³

1967

Biochemical Journal

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“…This is the case for all of the 'inner' S atoms of the thiosulphate molecule, but the 'outer'S atoms may initially be incorporated in endogenous sulphur compounds -in the detoxification of prussic acid this is the rhodanite sulphur (Eichler, 1950;Skarzynski et at, 1959;Szczepkowski et aI., 1961).…”

Section: Eliminationmentioning

confidence: 97%

Clinical Pharmacokinetics of Nitroprusside, Cyanide, Thiosulphate and Thiocyanate

Schulz¹

1984

Clinical Pharmacokinetics

170

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Sodium nitroprusside decomposes within a few minutes after intravenous infusion to form metabolites which are pharmacologically inactive but toxicologically important. Free cyanide, which represents 44% w/w of the sodium nitroprusside molar mass, is formed and must be detoxified in the body into thiocyanate using thisulphate as substrate. Nitroprusside penetrates cell membranes slowly. At therapeutic dose levels its distribution is probably mainly extracellular. Contact with the sulfhydryl groups in the cell walls, however, immediately initiates breakdown of the molecule. Sodium nitroprusside taken orally is not absorbed from the gastrointestinal tract to any appreciable extent. Cyanides in the body form prussic acid, which can rapidly penetrate mucous and cell membranes. In the blood, about 99% of the prussic acid binds to the methaemoglobin of erythrocytes. At normal physiological levels, however, the total body methaemoglobin of an adult human can only bind about 10mg of prussic acid; this is a small fraction of the amounts usually infused therapeutically as sodium nitroprusside. The endogenous detoxification of prussic acid exhibits zero-order kinetics. The limiting factor is a sulphur donor, principally thiosulphate, which is available in the body in only limited amounts. The rate of spontaneous detoxification of prussic acid in humans is only about 1 microgram/kg/min, corresponding to a sodium nitroprusside infusion of about 2 microgram/kg/min. This dose limit set by the prussic acid toxicity of sodium nitroprusside can, however, be increased considerably by simultaneous infusion of thiosulphate. A lack of thiosulphate can be detected early by a rise of the prussic acid concentration in the erythrocytes. Thiosulphate taken orally is not absorbed by the body. After intravenous infusion, its serum half-life is about 15 minutes. Most of the thiosulphate is oxidised to sulphate or is incorporated into endogenous sulphur compounds; a small proportion is excreted through the kidneys. Thiocyanate taken orally is completely absorbed by the body. In healthy persons its volume of distribution is approximately 0.25 L/kg and the serum half-life about 3 days; elimination is mainly renal. Thiocyanate toxicity does not represent a serious therapeutic problem with intravenous infusion of sodium nitroprusside.

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“…In recent years, studies have shown that the concentration and metabolic abnormalities of active sulfur-containing molecules are closely related to a variety of diseases. − For example, biothiol and hydrogen sulfide are closely related to cardiovascular diseases. The endogenous sulfur dioxide concentration is closely related to the occurrence and development of cancer. − Studying their metabolic pathways is conducive to effective intervention to prevent the occurrence and development of related major diseases.…”

Section: Introductionmentioning

confidence: 99%

A New Strategy: Distinguishable Multi-substance Detection, Multiple Pathway Tracing Based on a New Site Constructed by the Reaction Process and Its Tumor Targeting

et al. 2020

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In recent years, it has become a trend to employ organic molecular fluorescent probes with multireaction sites for the distinguishable detection and biological imaging of similar substances. However, the introduction of multireaction sites brought great challenges to organic synthesis, and at the same time, often destroyed the conjugated structure of the molecules, leading to an unsatisfactory fluorescence emission wavelength not conducive to practical application. As the eternal theme of life, metabolism goes on all the time. Metabolism is a series of ordered chemical reactions that occurs in the organism to maintain life. Chemical reactions in metabolism can be summarized as metabolic pathways. Simultaneous monitoring of different metabolic pathways of the same substance poses a lofty challenge to the probe. Here, we developed a new strategy: to construct new sites through the preliminary reactions between probes and some targets, which can be used to further distinguish among targets or detect their metabolites, so as to realize the simultaneous visualization tracer of multiple metabolic pathways. By intravenous injection, it revealed that the probe containing benzopyrylium ion can target tumors efficiently, and thiols are highly expressed in tumors compared to other tissues (heart, lung, kidney, liver, etc.). The consumption of thiols by the probe could not prevent tumor growth, suggesting that the tumor cure was not correlated with thiol concentration. The construction of new sites in the reaction process is a novel idea in the pursuit of multiple reaction sites, which will provide more effective tools for solving practical problems.

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Thiosulphate Metabolism in the Animal Organism

Cited by 25 publications

References 2 publications

[35S]thiosulphate oxidation by rat liver mitochondria in the presence of glutathione

[35S]thiosulphate oxidation by rat liver mitochondria in the presence of glutathione

Clinical Pharmacokinetics of Nitroprusside, Cyanide, Thiosulphate and Thiocyanate

A New Strategy: Distinguishable Multi-substance Detection, Multiple Pathway Tracing Based on a New Site Constructed by the Reaction Process and Its Tumor Targeting

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