BackgroundAcellular Pertussis vaccines against whooping cough caused by Bordetella pertussis present a much-improved safety profile compared to the original vaccine of killed whole cells. The principal antigen of acellular Pertussis vaccine, Pertussis Toxin (PT), must be chemically inactivated to obtain the corresponding toxoid (PTd). This process, however, results in extensive denaturation of the antigen. The development of acellular Pertussis vaccines containing PTd or recombinant PT (rPT) with inactivated S1, Filamentous Hemagglutinin (FHA), and Pertactin (PRN) has shown that the yield of PRN was limiting, whereas FHA was overproduced. To improve antigen yields and process economics, we have constructed strains of Bordetella pertussis that produce enhanced levels of both rPT and PRN.ResultsThree recombinant strains of Bordetella pertussis were obtained by homologous recombination using an allelic exchange vector, pSS4245. In the first construct, the segment encoding PT subunit S1 was replaced by two mutations (R9K and E129G) that removed PT toxicity and Bp-WWC strain was obtained. In the second construct, a second copy of the whole cluster of PT structural genes containing the above mutations was inserted elsewhere into the chromosome of Bp-WWC and the Bp-WWD strain was obtained. This strain generated increased amounts of rPT (3.77 ± 0.53 μg/mL) compared to Bp-WWC (2.61 ± 0.16 μg/mL) and wild type strain (2.2 μg/mL). In the third construct, a second copy of the prn gene was inserted into the chromosome of Bp-WWD to obtain Bp-WWE. Strain Bp-WWE produced PRN at 4.18 ± 1.02 μg/mL in the cell extract which was about two-fold higher than Bp-WWC (2.48 ± 0.10 μg/mL) and Bp-WWD (2.31 ± 0.17 μg/mL). Purified PTd from Bp-WWD at 0.8-1.6 μg/well did not show any toxicity against Chinese hamster ovary (CHO) cell whereas purified PT from WT demonstrated a cell clustering endpoint at 2.6 pg/well.ConclusionsWe have constructed Bordetella pertussis strains expressing increased amounts of the antigens, rPT or rPT and PRN. Expression of the third antigen, FHA was unchanged (always in excess). These strains will be useful for the manufacture of affordable acellular Pertussis vaccines.
ABSTRACTSeven distinctBacillus thuringiensissubsp.aizawaiintegrants were constructed that carried the chitinase (chiBlA) gene fromB. licheniformisunder the control of thecry11Aapromoter and terminator with and withoutp19andp20genes. The toxicity ofB. thuringiensissubsp.aizawaiintegrants against second-instarSpodoptera lituralarvae was increased 1.8- to 4.6-fold compared to that of the wild-type strain (BTA1). Surprisingly, the enhanced toxicity in some strains ofB. thuringiensissubsp.aizawaiintegrants (BtaP19CS,BtaP19CSter, andBtaCAT) correlated with an increase in toxin formation. To investigate the role of these genes in toxin production, the expression profiles of the toxin genes,cry1AaandchiBlA, as well as their transcriptional regulators (sigKandsigE), were analyzed by quantitative real-time RT-PCR (qPCR) from BTA1,BtaP19CS, andBtaCAT. Expression levels ofcry1Aain these two integrants increased about 2- to 3-fold compared to those of BTA1. The expression of the transcription factorsigKalso was prolonged in the integrants compared to that of the wild type; however,sigEexpression was unchanged. Western blot analysis of σEand σKshowed the prolonged accumulation of σEin the integrants compared to that of BTA1, resulting in the increased synthesis of pro-σKup toT17after the onset of sporulation in bothBtaP19CS andBtaCAT compared to that ofT13in BTA1. The results from qPCR indicate clearly that thecry1Aapromoter activity was influenced most strongly by σE, whereascry11Aadepended mostly on σK. These results on large-crystal toxin formation with enhanced toxicity should provide useful information for the generation of strains with improved insecticidal activity.
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