Yanti Y, Warnita, Reflin, Busniah M. 2018. Indigenous endophyte bacteria ability to control Ralstonia and Fusarium wiltdisease on chili pepper. Biodiversitas 19: 1532-1538. Bacterial wilt and Fusarium wilt caused by Ralstonia syzigii subsp. indonesiensisand Fusarium oxysporum f.sp. capsici (Foc), respectively are the most damaging vascular pathogens in chili pepper (Capsicum annuumL.) and many other crops limiting their production, worldwide. Various strategies have been developed to control wilt pathogensincluding the application of chemical pesticides, which generally considered as the most effective and fastest strategy for plant diseasemanagement. However, effective chemicals for wilt pathogens of chili pepper plants are not available, yet. Endophytic bacteriaconsidered as one of options to control vascular wilt disease because of its ability to live and colonize in internal roots of plants.Previous research has been done to select endophytic indigenous bacteria isolates which can promote growth rate of chili pepper. Thepurpose of the research was to identify the selected indigenous endophyte bacteria isolates acquired from our previous study using 16SrRNA identifications and to screen the selected endophytic indigenous bacteria to control both R. syzigii subsp. indonesiensis and Foc.Results from 16S r RNA analysis showed that all of 9 isolates were identified as Bacillus spp., such as Bacillus cereus ATCC 14579,Bacillus pseudomycoides strain NBRC 101232, Bacillus toyonensis strain BCT-7112, Bacillus thuringiensis strain ATCC 10792,Bacillus weihenstephanensis strain DSM 11821, Bacillus mycoides strain 273, Bacillus cereus strain NBRC 15305, Bacillusbingmayongensis strain FJAT-13831 and Bacillus manliponensis strain BL4-6. Our results showed that most of endophytic bacteriaisolates application could control both bacterial and Fusarium wilt diseases. Six out of nine isolates can suppress R. syzigii subsp.indonesiensis without developing any symptoms and five isolates could suppress symptoms of Foc. Isolates Bacillus pseudomycoidesstrain NBRC 101232 Bacillus thuringiensis strain ATCC 10792 and Bacillus mycoides strain 273 were potential for control Foc and R.syzigii subsp. indonesiensis in chili pepper.

Yanti Y, Warnita, Reflin. 2018. Short Communication: Development of selected PGPR consortium to control Ralstonia syzygii subsp. indonesiensis and promote the growth of tomato. Biodiversitas 19: xxxx. A microbial consortium is a group of different species of microorganisms and acts as a community. Combinations of biocontrol strains are expected to have a better result to suppress multiple plant diseases. Our previous research had selected four plant growth promoting rhizobacteria (PGPR) strains from chili (B. pseudomycoides strain NBRC 101232, B. cereus strain CCM 2010, Bacillus toyonensis strain BCT-7112, Serratia nematodiphila strain DZ0503SBS1) and three strains from tomato (Bacillus pseudomycoides strain NBRC 101232, Bacillus toyonensis strain BCT-7112, Bacillus thuringiensis strain ATCC 10792 ) which had ability to promote growth and control Ralstonia syzygii subsp. indonesiensis indigenously. The strains were used in the development of PGPR consortiums to increase their ability for biocontrol agent of Ralstonia syzigii subsp. indonesiensis and promoting the growth of tomato. Results showed that not all strains had good compatibility to grow together. Ten consortiums were developed based on their compatibilities. All consortiums exhibited the capability to reduce bacterial wilt disease development and also promote the growth of tomato. The consortium consisted of Serratia nematodiphila strain DZ0503SBS1, B. cereus strain CCM 2010, Bacillus aryabhattai strain B8W22 and Bacillus cereus strain IAM 12605 resulted in the best ability to reduce disease development and promote growth and yield of tomato.

Yanti Y, Warnita, Reflin, Nasution CR. 2018. Characterizations of endophytic Bacillus strains from tomato roots as growth promoter and biocontrol of Ralstonia solanacearum. Biodiversitas 19: 906-911. Bacterial wilt caused by Ralstonia solanacearum is the most damaging vascular pathogens in tomato and many other crops in tropical, subtropical and warm temperate areas of the world limiting its production. Biological agents such as Plant growth Promoting Rhizobacteria (PGPR) is considered as a potential biological control agent for the suppression of plant diseases such as bacterial wilt. Bacillus spp. are one of the most potential genera of PGPR group used for controlling pathogens and promoting plant growth because of their spore-forming ability which increases their adaptation to the environment. The aims of the research were to isolate Endophytic Bacillus isolates, to characterize its ability as plant growth promoter and pathogen controller, and to identify its molecular genetic using 16S rRNA. Bacillus strains were isolated from healthy tomato roots. All Bacillus spp. strains acquired from isolation were then screened directly on plants in completely randomized design experiments with 3 replications. All potential strains were screened and identified using 16S rRNA with 27F and 1492R primers. Results showed that out of 15 obtained isolates, 6 of them showed a good ability to both promote growth and control R. solanacearum. All isolates were identified as B. Pseudomycoides strain NBRC 101232, B. cereus strain CCM 2010, B. toyonensis strain BCT-7112, B. anthracis strain ATCC 14578, B. cereus strain JCM 2152 and B. cereus ATCC 14579.

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ABSTRAK Kelurahan Limau Manih Kota Padang merupakan kawasan pertanian yang cukup luas, dimana umumnya petani menanam padi, jagung dan sayur – sayuran. Salah satu sayuran yang dibudidayakan adalah cabai merah.  Permintaan terhadap cabai merah terus meningkat dan harganya berfluktuasi.  Flukasi harga yang tinggi menimbulkan masalah bagi masyarakat, apalagi cabai merah sangat dibutuhkan. Oleh karena itu perlu dilakukan penanaman cabai merah dalam pot di sekitar pekarangan rumah. Tujuan dari KKN-PPM adalah untuk mendorong empati mahasiswa dan dapat memberikan sumbangsih bagi penyelesaian persoalan yang ada di masyarakat dengan mengaplikasikan hasil-hasil penelitian untuk meningkatkan hasil cabai merah dalam pot. Metode yang diterapkan melalui pendekatan terhadap masyarakat petani dan pemuka masyarakat dengan menumbuhkembangkan dan memotivasi kelompok tani sehingga program KKN–PPM ini dapat diterima dengan baik oleh masyarakat.  Kegiatan yang dilakukan berupa : 1) penyuluhan, 2) pelatihan dan 3) demontrasi/ percontohan. Hasil yang dicapai dari kegiatan program KKN-PPM ini adalah : 1) Meningkatnya kepedulian dan empati mahasiswa terhadap permasalahan ekonomi dan sosial sehingga dapat meningkatkan pendapatan warga masyarakat; 2). Adanya respon positif yang diberikan warga masyarakat berupa partisipasi aktif dan antusias mengikuti kegiatan budidaya cabai merah dalam pot dari awal sampai akhir pelaksanaan. Kata kunci : Cabai merah, Pemberdayaan, Demonstrasi, Masyarakat, Pot ABSTRACT Limau Manih village is a large agricultural area, so farmers generally grow rice, maize and vegetables. One such vegetable is a red chili which is a daily necessity. The demand for red peppers continues to increase and their prices fluctuate. High price fluctuations cause problems for the community, especially chilli is needed. Therefore it is necessary to plant red pepper in pots around the yard of the house. The purpose of KKN-PPM is to encourage empathy students and can contribute to solving problems that exist in the community by applying the results of research to improve the results of red pepper in pots. Method applied by approach to farmer community and community leaders by cultivating and motivating farmer group so that KKN - PPM program can be accepted well by society. The activities are: 1) counseling, 2) training and 3) demonstration / demonstration. The results of this KKN-PPM program are: 1) Increased awareness and empathy of students on economic and social issues so as to increase the income of the community; 2). Positive response given by the community in the form of active and enthusiastic participation following the activity of red chili cultivation in pot from beginning to end of implementation                       . Keywords: red chili, empowerment, demontration, community, pot