Original scientific paper https://doi.org/10.2298/TSCI181115029GIn this study, the influence of slip flow and temperature jump on the entropy generation rate are investigated in rectangular microducts. The Knudsen numbers are considered in the range between 0.001 and 0.1, and the aspect ratio lies between 0 and 1. The dimensionless governing equations are solved numerically using Chebyshev spectral collocation method, and the dimensionless velocity and temperature gradients are employed in the entropy generation model. The influences of the dimensionless numbers including Bejan number and irreversibility distribution ratio on the entropy generation rates are investigated and discussed through surface plots and contour diagrams. It is demonstrated that the minimum entropy generation rate exists corresponding to an optimal aspect ratio for each dimensionless number. This minimum entropy generation rate depends upon the nature of dimensionless numbers.