The measurement of the atmospheric electric field is of great significance for the study of thunderstorm cloud charge models. Traditional electric field meters can only measure the vertical component of the atmospheric electric field, and thus it is difficult to invert the structure of the thunderstorm cloud. A three-dimensional atmospheric electric field meter was developed to simultaneously measure the horizontal and vertical components of the atmospheric electric field in this paper. The effective measurement linearly relates the measured electric field to the induced voltage, and the nonlinear equations of the three-dimensional atmospheric electric field and the thunderstorm cloud-charging model parameters were derived. The particle swarm optimization algorithm (PSO) and the three-dimensional atmospheric electric field were used to invert the thunderstorm clouds. Experimental observations of the three-dimensional electric field in a cloud during a thunderstorm were analyzed. Combined with the typical charged structure model, parameters such as the charge and relative distance of the thunderstorm cloud were determined. The results showed that the value of the inversion fitness function reached 0.7288, and the charge structure was even. The measurement of the three-dimensional atmospheric electric field provides a new means of observation for the study of atmospheric electricity.

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BackgroundPorphyromonas gingivalis (P. gingivalis), one of the main pathogenic bacteria involved in periodontitis, induces the expression of intercellular adhesion molecule − 1 (ICAM-1) and monocyte-endothelial cell adhesion. This effect plays a pivotal role in atherosclerosis development. Macrophage migration inhibitory factor (MIF) is a multifunctional cytokine and critically affects atherosclerosis pathogenesis. In this study, we tested the involvement of MIF in the P. gingivalis ATCC 33277-enhanced adhesive properties of endothelial cells.ResultsEndothelial MIF expression was enhanced by P. gingivalis ATCC 33277 infection. The MIF inhibitor ISO-1 inhibited ICAM-1 production in endothelial cells, and monocyte-endothelial cell adhesion was induced by P. gingivalis ATCC 33277 infection. However, the addition of exogenous human recombinant MIF to P. gingivalis ATCC 33277-infected endothelial cells facilitated monocyte recruitment by promoting ICAM-1 expression in endothelial cells.ConclusionsThese experiments revealed that MIF in endothelial cells participates in the pro-atherosclerotic lesion formation caused by P. gingivalis ATCC 33277 infection. Our novel findings identify a more detailed pathological role of P. gingivalis ATCC 33277 in atherosclerosis.

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Porphyromonas gingivalis ( P. gingivalis ) is an important pathogen that contributes to periodontal disease and causes infections that promote the progression of atherosclerosis. Our previous studies showed that macrophage migration inhibitory factor (MIF) facilitates monocyte adhesion to endothelial cells by regulating the expression of intercellular adhesion molecule-1 (ICAM-1) in P. gingivalis- infected endothelial cells. However, the detailed pathological role of MIF has yet to be elucidated in this context. To explore the functional receptor(s) of MIF that underlie its participation in the pathogenesis of atherosclerosis, we investigated the expression of the chemokine receptors CD74 and CXCR4 in endothelial cells, both of which were shown to be involved in the adhesion of monocytes to endothelial cells pretreated with P. gingivalis . Furthermore, the formation of a MIF, CD74, and CXCR4 ligand-receptor complex was revealed by our immunofluorescence staining and coimmunoprecipitation results. By interacting with the CD74/CXCR4 receptor complex, MIF may act as a crucial regulator of monocyte-endothelial cell adhesion and promote the atherosclerotic plaque formation induced by P. gingivalis .

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Cohesin, a multisubunit protein complex, is required for holding sister chromatids together during mitosis and meiosis. The recruitment of cohesin by the sister chromatid cohesion 2/4 (SCC2/4) complex has been extensively studied in Saccharomyces cerevisiae mitosis, but its role in mitosis and meiosis remains poorly understood in multicellular organisms, because complete loss-of-function of either gene causes embryonic lethality. Here, we identified a weak allele of Atscc2 (Atscc2-5) that has only minor defects in vegetative development but exhibits a significant reduction in fertility. Cytological analyses of Atscc2-5 reveal multiple meiotic phenotypes including defects in chromosomal axis formation, meiosis-specific cohesin loading, homolog pairing and synapsis, and AtSPO11-1-dependent double strand break repair. Surprisingly, even though AtSCC2 interacts with AtSCC4 in vitro and in vivo, meiosis-specific knockdown of AtSCC4 expression does not cause any meiotic defect, suggesting that the SCC2-SCC4 complex has divergent roles in mitosis and meiosis. SCC2 homologs from land plants have a unique plant homeodomain (PHD) motif not found in other species. We show that the AtSCC2 PHD domain can bind to the N terminus of histones and is required for meiosis but not mitosis. Taken together, our results provide evidence that unlike SCC2 in other organisms, SCC2 requires a functional PHD domain during meiosis in land plants.

The nonlinear synchronous full annular rub motion of a flexible rotor induced by the mass unbalance and the contact-rub force with rigid and flexible stator is studied analytically. The nonlinear property is due to the dry friction force between stator and rotor. The exact solutions of the synchronous full annular rub motion and its run speed regions are obtained. The stability of the synchronous full annular rub motion is discussed analytically. The stability criterion and the stability regions of the synchronous full annular rub motion are obtained. A simplified approximate criterion formula for dynamic stability is also derived under the conditions of large impact stiffness, small damping and small friction. The simplified criterion formula can be used conveniently in engineering and matches the real situations of industry.eMass eccentricity of the disk E i , υ i The Young's modulus and Poisson ratios of rotor and stator at the contact point F n or N Normal contact force between rotor and stator at contact point F t Tangential rub force between rotor and stator at the contact point h Clearance between rotor and stator k or k r Stiffness coefficient of the shaft at the disk position k s Stiffness coefficient of the stator at the contact position k p The equivalent stiffness coefficient of the contact force model m or m r Mass of rotor m s Mass of stator r or r r Absolute radial displacement of the geometric center C or O r of the disk r s Absolute displacement of the geometric center O s of the stator r st Relative distance between O s and O r r 0 , φ 0 Non-dimensional amplitude and phase angle of unbalance response of disk center before contact R Radius of disk of the rotor t Time u 0 Initial normal impact velocity x, y Generalized coordinates of the geometric center O s of the stator θ Angular position of the geometric center C or O r of the disk 580 G.F. Zhang et al.μ Friction coefficient ξ , ξ 1 , ξ 2 The non-dimensional embedded displacements of synchronous full annular rub at the contact point ω, ω r √ k/m, √ k r /m r , natural frequencies of rotor without rub ω s √ k s /m s , natural frequency of stator without rub Ω Spinning speed of the rotor Definition of non-dimensional quantities: e = e/ h,r = r/ h,R = R/ h,x = x/ h,ȳ = y/ h, r sr = r sr /h, τ = ωt, φ = Ωt − θ ,m s = m s /m r , k p = k p /k(or = k p /k r ),Ω = Ω/ω (or = Ω/ω r ), ω s = ω s /ω r , ς = c/(2 √ mk), ς r = c r /(2 √ m r k r ), ς s = c s /(2 √ m s k s ).