Knockdown of OPN gene expression suppresses colon cancer cell growth, adherence, invasion, and expression of angiogenic factors.

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MicroRNA-204 (miR-204) has been reported to be frequently downregulated in various types of cancer, including renal, brain, ovary, hematological and colon cancer. The present study, investigated the effects of miR‑204 on renal cell carcinoma. Following transfection of miR‑204, an MTT assay, cell migration assay, cell invasion assay, western blot analysis and luciferase assay were performed in renal cell carcinoma cell lines. It was demonstrated that miR‑204 inhibits cell proliferation, migration and invasion in 786‑O and A498 cells. To the best of our knowledge, this study is the first to demonstrate that miR‑204 directly targets SOX4 in renal cell carcinoma. These results suggested that miR-204 may have value as a marker for the early detection of tumor metastasis and a therapeutic target preventing the invasion of renal cell carcinoma.

Traffic congestion index reflects the state of traffic flow. The detection and analysis on traffic congestion index can be used to estimate the operation status of roads, to plan and organize road traffic for traffic managers, and to make the reasonable decisions of travelers to travel. The traffic conditions of several evaluation indexes were analyzed. Based on the theory of fuzzy mathematics, some membership functions of the evaluating indexes were designed. Three calculation methods of traffic congestion index were proposed. Their calculation results were compared mutually. The conclusion revealed that using saturation calculated by the corresponding service level of traffic congestion index not well reflect the traffic situation, what’s more, travel speed is used to calculate the congestion index of the first method. Using comprehensive parameters can calculate the congestion index of the third method. Both them are roughly similar and in line with the actual traffic phenomenon.