A full-length cDNA clone encoding an 866 bp-length glutathione peroxidase protein (NnGPX) was isolated from lotus (Nelumbo nucifera L.). The deduced amino acid sequence of the NnGPX gene had significant homology with ATGPX6. A 3D structural model of the NnGPX was constructed by homology modeling. The cloned NnGPX gene was expressed in Escherichia coli, and a fusion protein of about 40 kDa was detected after isopropyl thiogalactoside induction. Under different concentrations of Na2SeO3 treatments, NnGPX was found to be an enzyme that does not contain selenium. Real-time PCR analysis showed that the NnGPX gene was expressed in all organs of lotus, and its high expression mainly occurred in organs with active metabolisms. NnGPX transcript increased remarkably in response to cold, heat, mechanical damage, and salt treatment. Subsequently, the NnGPX gene was introduced in Oryza sativa cv. Yuetai B. PCR results verified the integration of this gene into the genome of rice and reverse transcription-PCR verified that this gene had been expressed in transgenic rice. The transgenic plants were significantly more tolerant to salt stress compared with the wild-type.
Under dehumidifying conditions, the condensed water will directly affect the heat transfer and resistance characteristics of a fin-and-tube heat exchanger. The geometrical form of condensed water on fin surfaces of three different fin materials (i.e., copper fin, aluminum fin, and aluminum fin with hydrophilic layer) in a fin-and-circular-tube heat exchanger was experimentally studied in this paper. The effect of the three different fin materials on heat transfer and friction performance of the heat exchanger was researched, too. The results show that the condensation state on the surface of copper fin and aluminum fin are dropwise condensation. The condensation state on the surface of the aluminum fin with the hydrophilic layer is film condensation. For the three different material fins, increasing the air velocity (ua,in) and relative humidity (RHin) of the inlet air can enhance the heat transfer of the heat exchanger. Friction factor (f) of the three different material fins decreases with the increase of ua,in, however, increases with the increase of RHin. At the same ua,in or RHin, Nusselt number (Nu) of the copper fin heat exchanger is the largest and Nu of the aluminum fin with hydrophilic layer is the smallest, f of the aluminum fin heat exchanger is the largest and f of the aluminum fin with hydrophilic layer is the smallest. Under the identical pumping power constrain, the comprehensive heat transfer performance of the copper fin heat exchanger is the best for the studied cases.
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