The genomic organisation of the seven cultivated Vigna species, V. unguiculata, V. subterranea, V. angularis, V. umbellata, V. radiata, V. mungo and V. aconitifolia, was determined using sequential combined PI and DAPI (CPD) staining and dual-colour fluorescence in situ hybridisation (FISH) with 5S and 45S rDNA probes. For phylogenetic analyses, comparative genomic in situ hybridisation (cGISH) onto somatic chromosomes and sequence analysis of the internal transcribed spacer (ITS) of 45S rDNA were used. Quantitative karyotypes were established using chromosome measurements, fluorochrome bands and rDNA FISH signals. All species had symmetrical karyotypes composed of only metacentric or metacentric and submetacentric chromosomes. Distinct heterochromatin differentiation was revealed by CPD staining and DAPI counterstaining after FISH. The rDNA sites among all species differed in their number, location and size. cGISH of V. umbellata genomic DNA to the chromosomes of all species produced strong signals in all centromeric regions of V. umbellata and V. angularis, weak signals in all pericentromeric regions of V. aconitifolia, and CPD-banded proximal regions of V. mungo var. mungo. Molecular phylogenetic trees showed that V. angularis and V. umbellata were the closest relatives, and V. mungo and V. aconitifolia were relatively closely related; these species formed a group that was separated from another group comprising V. radiata, V. unguiculata ssp. sesquipedalis and V. subterranea. This result was consistent with the phylogenetic relationships inferred from the heterochromatin and cGISH patterns; thus, fluorochrome banding and cGISH are efficient tools for the phylogenetic analysis of Vigna species.

Frost damage is a common durability problem for concrete structures in cold and wet regions, and in many cases, the frost damage is coupled with fatigue loadings such as the traffic loads on bridge decks or pavements. In this paper, to investigate the basic fatigue behavior of concrete materials affected by frost damage, a mesoscale approach based on Rigid Body Spring Method (RBSM) has been developed, of which the concrete material can be divided into three parts: mortar, coarse aggregate and interfacial transition zone (ITZ) between them. First, the cyclic constitutive laws are developed at normal and shear directions for mortar and ITZ, and verified with the existing experimental data in compression and tension fatigue. Then, several levels of frost damage are introduced by different numbers of freeze-thaw cycles (FTCs), and finally, the static tests and fatigue tests are conducted using the frost damaged concrete. The simulation results on the static strength and fatigue life show a good agreement with experimental data, and found that as the frost damage level (irreversible plastic deformation) increases, not only the static strength, but also the fatigue life at each stress level will decrease. The S-N curves of frost damaged concrete still follow a linear relationship but with bigger slopes, and the frost damaged concrete will become more ductile under fatigue loadings.

This study examines the spillover effects of foreign direct investment (FDI) on green technology progress rate (as measured by the green total factor productivity). The analysis utilizes two measures of FDI, labor-based FDI and capital-based FDI, and separately investigates four sets of industry classifications—high/low discharge regulation and high/low emission standard regulation. The results indicate that in the low discharge regulation and low emission standard regulation industry, labor-based FDI has a significant negative spillover effect, and capital-based FDI has a significant positive spillover effect. However, in the high-intensity environmental regulation industry, the negative influence of labor-based FDI is completely restrained, and capital-based FDI continues to play a significant positive green technological spillover effects. These findings have clear policy implications: the government should be gradually reducing the labor-based FDI inflow or increasing stringency of environmental regulation in order to reduce or eliminate the negative spillover effect of the labor-based FDI.

A series of environmentalegeological problems have been caused by over-exploitation of deep groundwater (i.e., confined aquifer water) in the North China Plain. In order to better understand the status of deep groundwater over-exploitation and the resultant environmentalegeological problems on a regional scale, the over-exploitation of groundwater has been assessed by way of the groundwater exploitation potential coefficient (i.e., the ratio of exploitable amount of deep groundwater to current exploitation), cumulative land subsidence, and long-term average lowering rate of the groundwater table. There is a good correlation among the results calculated by the different methods. On a regional scale, deep groundwater has been over-exploited and there is no further exploitation potential under the current conditions. The groundwater exploitation degree index takes the exploitation in 2003 as the reference for the calculations, so the results mainly reflect the degree of current groundwater exploitation. The results of over-exploitation of deep groundwater obtained by land subsidence data and long-term average rate of depression of the water table mainly reflect environmentalegeological problems caused by exploitation of deep groundwater. ª 2011, China University of Geosciences (Beijing) and Peking University. Production and hosting by Elsevier B.V. All rights reserved.

The incidence of PTEN mutations is relatively high in patients with sporadic breast cancer in the region of Yunnan, China and exists at the early stage of breast cancer development. The PTEN mutations have significant effect on the expression silencing of PTEN protein indicating the important role of PTEN mutation in carcinogenesis of breast cancers.

There are obvious differences between different types of environmental regulation, which are manifested in different environmental protection tendencies and goals, and there are also significant differences in policy implementation. Therefore, it is an urgent empirical problem to quantitatively evaluate the impact of heterogeneous environmental regulation on the upgrading of industrial structures. Therefore, on the basis of measuring the level of industrial structure upgrading, this paper empirically tests the impact of heterogeneous environmental regulation on industrial structure upgrading. The results show that the upgrading level of China's industrial structure increased year by year from 2000 to 2018. The three types of environmental regulatory measures have effectively promoted the upgrading of the industrial structure, and the market-incentive environmental regulation (MER) has a significantly higher role in the advancement of the industrial structure than the command-controlled environmental regulation (CER) and the voluntary public participation environmental regulation (VER). With the improvement of regional economic development level, the three types of environmental regulation have gradually become more prominent in promoting the upgrading of industrial structure. In the mechanism test, it is found that technological innovation has a partial mediating effect in the process of CER affecting the upgrading of industrial structure, MER and public VER have a complete mediating effect, while foreign direct investment (FDI) has only a partial mediating effect in the process of the three types of environmental regulation affecting industrial advancement, and there is no mediating effect in the process of influencing the rationalization of industrial structure.

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The genus Cyphellophora includes human- and plant-related species from mammal skin and nails, plant materials, and food. On the basis of analysis of ITS, LSU, TUB2 and RPB1 data and morphological characters, three new species, Cyphellophora phyllostachysdis, C. artocarpi and C. musae, associated with sooty blotch and flyspeck disease, were added to this genus. The 2D structure of ITS1 and ITS2 confirmed this taxonomic status. Pathogenicity tests on apple fruit indicated that C. artocarpi could be a sooty blotch and flyspeck pathogen of apple.