No abstract

High-sensitive measurement of radio-frequency (RF) electric field is available via the electromagnetically induced transparency (EIT) effect of Rydberg atom at room-temperature, which has been developed to be a promising atomic RF receiver. In this Letter, we investigate the credibility of the digital communication via this quantum-based antenna over the entire continuously tunable RF-carrier. Our experiment shows that digital communication at a rate of 500 kbps performs reliably within a tunable bandwidth of 200 MHz at carrier 10.22 GHz and a bit error rate (BER) appears out of this range, for example, the BER runs up to 15% at RF-detuning ±150 MHz. In the measurement, the time-variant RF field is retrieved by detecting the density of the probe laser at the center frequency of RF-induced symmetric or asymmetric Autler-Townes splitting in EIT. Prior to the digital test, we have studied the RF-receiving quality versus the physical ambiance and found that a choice of linear gain response to the RF-amplitude can suppress the signal distortion and the modulating signal is able to be decoded as fast as up to 500 kHz in the tunable bandwidth. Our checkout consolidates the physical foundation for a reliable communication and spectrum sensing over the broadband RFE-field signal in free-space can be captured by measuring the transmission of a probe laser in a condition of a Rydberg EIT. Owing to unique advantages of free-space RF field sensing, the quantum receiver has great significance compared with conventional electronics-based receivers, including but not limited to the weak signal, long-distance communication in free space or via a fiber link. All the principle experiments of communication were performed over carrier of an optimized resonant frequency of Rydberg states [11][12][13].

Abstract:Since the Chinese government carried out the reform and opening up policy, Xishuangbanna Dai Autonomous Prefecture has experienced rapid urbanization and dramatic land use change. This research aims at analyzing urban expansion in Xishuangbanna and its impact on the land use pattern using combined methods, including radar graph, the gradient-direction method and landscape metrics. Seven land use maps from 1976 to 2015 were generated and analyzed, respectively. The results showed that urban and rubber expanded rapidly, while forest decreased during the last 40 years. The city proper, the county town of Menghai and the county town of Mengla showed the most significant and fastest urban expansion rates. In response to rapid urban expansion, land use types outside urban areas changed dramatically. In Jinghong and Mengla, urban areas were usually surrounded by paddy, shrub, rubber and forest in 1976, while most areas were dominated by rubber by 2015. With the development of Xishuangbanna, landscape diversity increased along urban-rural gradients, but decreased in some key urban areas. Urban expansion slightly reduced the connectivity of forest and increased agglomeration of rubber at the same time. Based on the analyses above, we moved forward to discuss the consequences of urban expansion, rubber plantations and land fragmentation.

Gastric cancer (GC) is a prevalent malignant cancer of digestive system, identification of novel diagnostic and prognostic biomarkers for GC is urgently demanded. The aim of this study was to determine potential long noncoding RNAs (lncRNAs) associated with the pathogenesis and prognosis of GC. Raw noncoding RNA microarray data (GSE53137, GSE70880, and GSE99417) was downloaded from Gene Expression Omnibus (GEO) database. Differentially expressed genes between GC and adjacent normal gastric tissue samples were screened by an integrated analysis of multiple gene expression profile after gene reannotation and batch normalization. Differentially expressed genes were further confirmed by The Cancer Genome Atlas (TCGA) database. Competing endogenous RNA (ceRNA) network, Gene Ontology term and Kyoto Encyclopedia of Genes and Genomes pathway, survival analysis were extensively applied to identify hub lncRNAs and discover potential biomarkers related to diagnosis and prognosis of GC. In total of 246 integrated differential genes including 15 lncRNAs and 241 messenger RNAs (mRNAs) were obtained after intersections of differential genes between GEO and TCGA database. ceRNA network comprised of three lncRNAs (UCA1, HOTTIP, and HMGA1P4), 26 microRNAs (miRNAs) and 72 mRNAs. Functional analysis revealed that three lncRNAs were mainly dominated in cell cycle and cellular senescence. Survival analysis showed that HMGA1P4 was statistically related to the overall survival rate. For the first time, we identified that HMGA1P4, a target of miR‐301b/miR‐508, is involved in cell cycle and senescence process by regulating CCNA2 in GC. Finally, the expression levels of three lncRNAs were validated to be upregulated in GC tissues. Thus, three lncRNAs including UCA1, HOTTIP, and HMGA1P4 may contribute to GC development and their potential functions might be associated with the prognosis of GC.

Zircon water content is an important physicochemical parameter for many geological processes, yet its measurement by the secondary ion mass spectrometry (SIMS) technique is hampered by the lack of suitable reference materials and high water background, especially if large-geometry (LG)-SIMS is used.

Betulinic acid (BA) exhibits cytotoxic activity against some cancer cells. However, the molecular mechanism of BA against CRC cells was little reported. Here, we proved that BA elicited CRC cells' growth inhibition and apoptosis in a dose-dependent manner. In addition, BA treatment induced autophagy via inhibiting the AKT-MTOR signaling pathway. Inhibition of autophagy by either administration of autophagic inhibitor chloroquine or siRNA-mediated knockdown of ATG5 could augment BA-induced apoptotic cell death as well as inhibition of cell proliferation. Moreover, we found that p53 was firstly activated by short exposure to BA and then was rapidly degraded via the ubiquitin-mediated degradation pathway in both wtp53 and mutp53 CRC cells. Notably, more preferential cytotoxicity of BA was obtained in mutp53 cells (IC50 values: HT29, 125 μM; SW480, 58 μM) rather than wtp53 cells (IC50 values: HCT116, 178 μM). Further experiments demonstrated that siRNA-mediated p53 knockdown attenuated BA-induced autophagy, and forced overexpression of p53 augmented BA-induced autophagy, indicating that p53-enhanced BA-induced autophagy. Moreover, BA enhanced the sensitivity of mutp53 cells to chemotherapy drugs such as 5-FU and ADR by degradation of mutp53. Overall, our study proved that BA could induce CRC cell death by inducing apoptosis and reduce the overaccumulation of BA-induced protective autophagy by degrading wtp53 and mutp53 dependent on the ubiquitin-mediated degradation pathway to achieve killer effect, suggesting that BA might serve as a novel desirable drug for mutp53 cancer therapy.

No abstract

Background Breast cancer (BC) is a common malignant tumor and its incidence and mortality rates are ranked first among female cancers. So far, there has been no effective biomarkers for BC prognosis. Methods The DNA methylation data of BC was downloaded from The Cancer Genome Atlas (TCGA), Gene Expression Omnibus, and Functional ANnoTation of The Mammalian Genome databases. The RNA‐Seq data and clinical information of patients were downloaded from TCGA. R packages edgeR and minfi were used for differentially methylated genes (DMGs) screening. Then, the DMGs were collected for gene ontology and Kyoto Encyclopedia of Genes and Genomes analysis by the online tool database for annotation, visualization and integrated discovery (DAVID) and Reactome. Cox regression analysis was used to screen candidate differentially methylated sites (DMSs) for BC prognosis. Logrank test was used to explore the correlation between DMSs and survival time. Correlation analysis was used to investigate the correlation between DNA methylation and gene expression. Results We identified 276 DMGs which contained 1454 DMSs in those three datasets. Also, six DMGs that contained seven DMSs were identified by Cox regression analysis. Interestingly, their expression levels were negatively correlated with the DNA methylation level and not affected by age, subtypes, or tumor stages. Conclusions We proposed that these seven differentially DNA methylation sites can be used as a novel prognostic biomarker for BC area under curve (AUC) = 0.74), which may facilitate research and benefit the clinical treatment of BC.