This study studied the best levels of bioethanol from a combination of cassava peel and pineapple peel mixtures with 6 gram yeast mass and 72 hours, 96 hours and 120 hours fermentation time, to determine the optimal fermentation time and find ethanol levels according to SNI. This research was carried out by hydrolysis or cassava peel and pineapple peel using aquades for 30 minute to break the molecule into two parts, then carried out the fermentation process using Saccharomyces Cerevisae (yeast) and the distillation process using a destilator to obtain ethanol for fermentation then tested with a Refractometer to determine whether there is an ethanol level formed from the distillation process. The best sample selected was then tested for ethanol content using the Gas Chromatography tool. The results of this study are known by using the Gas Chromatography tool to determine the ethanol content contained in the distilled sample, the highest ethanol content of each combination. So that the best ethanol yield from a combination of 100% cassava peel -0% Pineapple Peel with a duration of 120 hours fermentation producing ethanol of 89.81% is still not included in the SNI category. I. PENDAHULUANMenipisnya cadangan bahan bakar fosilsdan semakin meningkatnya populasi manusiamsangat berpengaruh terhadap kebutuhanmenergi bagi kelangsungan hidup manusia. Sejak limaatahun terakhir, Indonesia mengalami dampak penurunan produksi minyak nasional. padahal dengan menambahnya jumlah penduduk indonesia, semakin meningkat pulaakebutuhan akannsarana transportasi dan aktivitas industri. Hal ini berakibat pada peningkatan kebutuhan dan konsumsi bahan bakar minyak.Oleh karena ituvsudah saatnyaauntuk Indonesiaamencari alternatif lain, dimana sumber energi fosil yang sifatnya tidak terbarukannberalih ke sumber energi berbahan baku nabatiyyang sifatnya terbarukan. Sebagai negara agraris danntropis, Indonesia telah dianugerahi kekayaannalam yang melimpah yang dapat dimanfaatkan sebagai bioenergi. Bahan bakar berbasis nabati salah satu contohnya adalah bioetanol. Bioetanol merupakan senyawa alkohol yang diperolehmlewat proses fermentasi biomassa dengan bantuan mikroorganisme. Bioetanol dapat dibuattdari sumber daya hayatiyyang sangat melimpah di Indonesia contohnyaddari bahan-bahan bergula atau pati seperti Kuluit singkong, Kulit nanas, tebu, nira, sorgum, ubi jalar, dan lain-lain. Semuanya merupakan tanaman yang memiliki karbohidrat dan mudah ditemukan di indonesia karena keadaan tanah dan iklim yang sangat mendukung.
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