The aim of this study which conducted in Al-Diwaniyah province during the period from October (2021) to April (2022) was to determine the prevalence of Eimeria infection in goat and study the effect of some epidemiological factor such as sex ,age and months on the infection rates addition to that and molecular identification of Eimeria. Two hundred and ten fecal sample were collected, from goat in four different regions in Al-Diwaniyah province included (Al-Shamiya, Al-Sunyih, Ghamas and Al-Mohanawih) at one visit per a week for each regions. The results of microscopic examination showed that 169(80.48%) of goat were infected. According to morphological study, eight Eimeria species were recorded which include E. arloingi, E. alijevi, E. ninakohlyakimovae, E. christenseni, E. jolchijevi, E. hirci, E. caprovina and E. caprina. The results showed that, Eimeria arloingi were recorded at highest rate (22.86%), while E. caprina was observed at lowest rate (3.33%). Significant (P<0.01) differences was found between prevalence of infections according to Eimeria species. Out of 120 fecal sample were collected from goat female and 90 fecal samples were collected from male which examined as Eimeria species, the results showed that, 99(82.5%) females and 70(77.78%) males were confirmed to be infected.
The aim of this study which conducted in Al-Diwaniyah province during the period from October (2021) to April (2022) was to determine the prevalence of Eimeria infection in goat and study the effect of some epidemiological factor such as sex ,age and months on the infection rates addition to that and molecular identification of Eimeria. Two hundred and ten fecal sample were collected, from goat in four different regions in Al-Diwaniyah province included (Al-Shamiya, Al-Sunyih, Ghamas and Al-Mohanawih) at one visit per a week for each regions. The results of microscopic examination showed that 169(80.48%) of goat were infected. According to morphological study, eight Eimeria species were recorded which include E. arloingi, E. alijevi, E. ninakohlyakimovae, E. christenseni, E. jolchijevi, E. hirci, E. caprovina and E. caprina. The results showed that, Eimeria arloingi were recorded at highest rate (22.86%), while E. caprina was observed at lowest rate (3.33%). Significant (P<0.01) differences was found between prevalence of infections according to Eimeria species. Out of 120 fecal sample were collected from goat female and 90 fecal samples were collected from male which examined as Eimeria species, the results showed that, 99(82.5%) females and 70(77.78%) males were confirmed to be infected.
The aim of this study which conducted in Al-Diwaniyah province during the period from October (2021) to April (2022) was to study histopathological examination of intestine and molecular identification with phylogenetic analysis of Eimeria. A total of 25 tissue samples (small intestine and large intestine) of goat were collected from slaughter houses in Al-Diwaniyah province. Histopathological study of the intestine revealed immature oocyst of the parasite in the colon, and the reproductive stages of the parasite were observed, represented by the presence of female (macrogametes) and male (microgametes), in addition to observing some histopathological changes represented by the presence of infiltration of lymphocyte and eosinophil of villi in goat. Genomic DNA was extracted from 100 goat´s fecal samples and18S rRNA gene of Eimeria was amplified by polymerase chain reaction. PCR technique showed that, out of 100 goat´s fecal samples 87(87%) were positive for 18S rRNA gene of Eimeria. Fifteen PCR positive product of local Eimeria isolates were sent to Bioneer company in Korea for detected the DNA sequence and compared it with that of other Eimeria published in GenBank.
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