Cervical cancer is one of the highest causes of death in Indonesian women caused by mutations of normal cervical cells turning into abnormal cells. Treatment of cervical cancer causes significant side effects with a relatively long treatment period, so many researchers are exploring various natural ingredients from marine ecosystems as candidates for anticancer drugs. One of them is the sea sponge Petrosia sp. containing various secondary metabolites, one of which is an alkaloid that has a cytotoxic effect on cancer cell lines. This study aims to determine the cytotoxic activity of the ethanol extract of Petrosia sp. in HeLa cervical cancer cells. The ethanol extract of Petrosia sp. obtained by maceration using 96% ethanol and obtained extract as much as 55.9 g and yield value of 2.94%. Cytotoxic activity test was carried out in vitro using the Presto Blue method with varying concentrations of ethanol extract 7.81 ppm; 15.62 ppm; 31.25 ppm; 62.5 ppm; 125 ppm; 250 ppm; 500 ppm; and 1000 ppm. The cytotoxic parameter used was IC50 which was determined using the GraphPad Prism software version 5. The IC50 value of the marine sponge extract Petrosia sp. of 97.20 ppm or 97.20 g/ml with an active category as cervical anticancer. Cervical cancer is one of the highest causes of death in Indonesian women caused by mutations of normal cervical cells turning into abnormal cells. Treatment of cervical cancer causes significant side effects with a relatively long treatment period, so many researchers are exploring various natural ingredients from marine ecosystems as candidates for anticancer drugs. One of them is the sea sponge Petrosia sp. containing various secondary metabolites, one of which is an alkaloid that has a cytotoxic effect on cancer cell lines. This study aims to determine the cytotoxic activity of the ethanol extract of Petrosia sp. in HeLa cervical cancer cells. The ethanol extract of Petrosia sp. obtained by maceration using 96% ethanol and obtained extract as much as 55.9 g and yield value of 2.94%. Cytotoxic activity test was carried out in vitro using the Presto Blue method with varying concentrations of ethanol extract 7.81 ppm; 15.62 ppm; 31.25 ppm; 62.5 ppm; 125 ppm; 250 ppm; 500 ppm; and 1000 ppm. The cytotoxic parameter used was IC50 which was determined using the GraphPad Prism software version 5. The IC50 value of the marine sponge extract Petrosia sp. of 97.20 ppm or 97.20 g/ml with an active category as cervical anticancer.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.