Japanese encephalitis (JE) is a global public health issue that has spread widely to more than 20 countries in Asia and has extended its geographic range to the south Pacific region including Australia. JE has become the most important cause of viral encephalitis in the world. Japanese encephalitis viruses (JEV) are divided into five genotypes, based on the nucleotide sequence of the envelope (E) gene. The Muar strain, isolated from patient in Malaya in 1952, is the sole example of genotype V JEV. Here, the XZ0934 strain of JEV was isolated from Culex tritaeniorhynchus, collected in China. The complete nucleotide and amino acid sequence of XZ0934 strain have been determined. The nucleotide divergence ranged from 20.3% to 21.4% and amino acid divergence ranged from 8.4% to 10.0% when compared with the 62 known JEV isolates that belong to genotype I–IV. It reveals low similarity between XZ0934 and genotype I–IV JEVs. Phylogenetic analysis using both complete genome and structural gene nucleotide sequences demonstrates that XZ0934 belongs to genotype V. This, in turn, suggests that genotype V JEV is emerging in JEV endemic areas. Thus, increased surveillance and diagnosis of viral encephalitis caused by genotype V JEV is an issue of great concern to nations in which JEV is endemic.

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Some new discoveries of the family Epicopeiidae Swinhoe, 1892 from China are reported. A new species, Mimaporiaowadai Huang & Wang, sp. n. is described from W. Sichuan. Burmeia Minet, 2003 and Psychostrophiaendoi Inoue, 1992 are reported as new to China, with the female genitalia of the former described for the first time. The females of Psychostrophiaendoi Inoue, 1992 and Deuveiabanghaasi Hering, 1932 are reported for the first time. Adults and genitalia of all species aforementioned are illustrated.

The chestnut tiger butterfly, Parantica sita (Kollar) (Lepidoptera: Nymphalidae: Danainae), occurs in Asia, along the Himalayas, and into the Malayan region. Previous studies found three types of mitogenomes with substantial genetic divergence in samples from China. To clarify the level of differentiation within P. sita, we investigated both molecular data and morphological features in 429 individuals from China. Upon examination, mitochondrial cytochrome oxidase subunit I (COI) sequences showed three substantially diverged haplotype groups. Based on microsatellite genotypes, the samples divided into three clusters that were consistent with the COI haplotype groups. With that genetic data, we named three distinguishable P. sita lineages: PS-A, PS-B, and PS-C. We also found obvious morphological differences in wing color, male sex brand, and genitalia structures among the three lineages. According to the published structure of male genitalia, that of PS-A is identical to that of P. s. sita, and that of PS-B is identical to that of P. pedonga. Based on all the results, we tentatively propose dividing P. sita into three species: PS-A (the former P. s. sita) is the typical Parantica sita [Kollar, (1844)], mainly distributed in southwestern China; PS-C (the former P. s. niphonica) is elevated to full species as Parantica niphonica (Moore, 1883), distributed in Taiwan Island and Japan; and PS-B will be Parantica pedongaFujioka, 1970, mainly distributed in Tibet and western Sichuan. Divergence time estimates showed that PS-A separated from the PS-B + PS-C clade about 8.79 million years ago (Ma), when the Hengduan Mountains underwent an appreciable elevation increase, isolating the Tibet population from the others. PS-B and PS-C diverged about 4.87 Ma, in accord with the formation of Taiwan Island mountains. The founder effect may explain why PS-C’s genetic diversity is lower than that of the other clades.

The collection of caterpillar fungus accounts for 50–70% of the household income of thousands of Himalayan communities and has an estimated market value of $5–11 billion across Asia. However, Himalayan collectors are at multiple economic disadvantages compared with collectors on the Tibetan Plateau because their product is not legally recognized. Using a customized hybrid-enrichment probe set and market-grade caterpillar fungus (with samples up to 30 years old) from 94 production zones across Asia, we uncovered clear geography-based signatures of historical dispersal and significant isolation-by-distance among caterpillar fungus hosts. This high-throughput approach can readily distinguish samples from major production zones with definitive geographical resolution, especially for samples from the Himalayan region that form monophyletic clades in our analysis. Based on these results, we propose a two-step procedure to help local communities authenticate their produce and improve this multi-national trade-route without creating opportunities for illegal exports and other forms of economic exploitation. We argue that policymakers and conservation practitioners must encourage the fair trade of caterpillar fungus in addition to sustainable harvesting to support a trans-boundary conservation effort that is much needed for this natural commodity in the Himalayan region.

Background: To compare qualitative and quantitative superb microvascular imaging (SMI) and determine the value of SMI in the diagnosis of thyroid nodules (TNs) ≤10 mm based on the Chinese Thyroid Imaging Reporting and Data System 4 (C-TIRADS 4). Methods: From October 2020 to June 2022, 106 patients with 109 C-TIRADS 4 (C-TR4) TNs (81 malignant, 28 benign) at the Peking Union Medical College Hospital were included. Qualitative SMI reflected the vascular pattern of the TNs and quantitative SMI was recorded by the vascular index (VI) of the nodules. Results: The VI was significantly higher in malignant nodules versus benign nodules both in the longitudinal (19.9±11.4 vs. 13.8±10.6, P=0.01) and transverse (20.2±12.1 vs. 11.3±8.7, P=0.001) sections. The area under the curve (AUC) of qualitative and quantitative SMI did not show a statistical difference in the longitudinal {0.657 [95% confidence interval (CI): 0.560-0.745] vs. 0.646 (95% CI: 0.549-0.735), P=0.79} and transverse [0.696 (95% CI: 0.600-0.780) vs. 0.725 (95% CI: 0.632-0.806), P=0.51] sections. Next, we combined qualitative and quantitative SMI to upgrade and downgrade the C-TIRADS classification. If a C-TR4B nodule had VIsum >12.2 or intra-nodular vascularity, the original C-TIRADS was upgraded to C-TR4C. If a C-TR4C or C-TR4B nodule manifested VIsum ≤12.2 and no intra-nodular vascularity, the original C-TIRADS was downgraded to C-TR4A. As a result, 18 C-TR4C nodules were downgraded to C-TR4A and 14 C-TR4B nodules were upgraded to C-TR4C. The new model of SMI + C-TIRADS showed high sensitivity (93.8%) and accuracy (79.8%). Conclusions: There is no statistical difference between qualitative and quantitative SMI in the diagnosis of C-TR4 TNs. The combination of qualitative and quantitative SMI may have the potential to manage diagnosis of C-TR4 nodules.

The genus Nola was established with the western Palaearctic species Noctua cucullatella (Linnaeus, 1758) described from Austria as its type species. Nola occurs in all continents except the Antarctica and contains more than 200 valid Eurasian and North African taxa (László et al., 2014). The genus Nola can be easily distinguished from other genera of Nolinae with the distinctive features as the degenerate uncus, the divided valva with the ventral valval lobe bearing the harpe and a curved carina process in male genitalia. In our recent survey, we report a new species Nola senmuzhaensis sp. nov. and a new record of Nola sikkima (Moore, 1888) from Tibet, China. Adults and genitalia are illustrated. The type specimens are deposited in Southwest University of Science and Technology (SWUST).