ABSTRACT. Liver fatty acid-binding protein (L-FABP) and liver bile acid-binding protein (L-BABP), in the liver intra-cytoplasm of chicken, are members of the fatty acid-binding protein subfamily. This study was designed to analyze and compare L-FABP and L-BABP expression levels between fat and lean lines in chicken liver tissue, and to determine the relationship between their expression and lipid metabolism. Realtime polymerase chain reaction (PCR) and Western blotting were used to detect the mRNA and protein expression in liver tissue between the lean and fat lines. Real-time PCR showed that L-FABP mRNA expression in fat male chickens was higher than that in lean male chickens at 2, 3, 4, 5, 6, and 10 weeks of age (P < 0.05), and L-BABP mRNA expression in fat male chickens was higher than that in lean male chickens at 1, 2, 3, 4, 8, and 10 weeks of age (P < 0.05). Western blotting showed that the L-FABP protein expression in fat male chickens was higher than that in lean male chickens at 3, 5, 6, and 7 weeks of age (P < 0.05), and L-BABP protein expression in fat male chickens was higher than that in lean male chickens at 3, 4, 5, and 6 weeks of age (P < 0.05). These results suggested that chicken L-FABP and L-BABP affect abdominal fat deposition through differences in their expression level, and the possible Expression of chicken L-FABP and L-BABP mechanism is that a high expression level of L-FABP and L-BABP leads to a high lipogenesis rate and, ultimately, to lipid deposition.
ObjectivesDiabetic nephropathy (DN) is one of the most severe diabetic complications and is becoming a worldwide epidemic, accounting for approximately one-third of all cases of end-stage renal disease. In this study, we investigated its influence on Th1/Th2 cells balance, inflammation and lipid metabolism to assess whether Danggui Shaoyao Powder (DSS) ameliorates DN.MethodsWistar rats were randomly divided into control group, model group, DSS group and benazepril group. The model group was induced by injection intraperitoneally with streptozotocin after high-calorie foods were given for 1 month. Animals were treated orally with saline, DSS and benazepril daily for 8 weeks. At the end of 8 weeks, blood glucose, 24 hour urinary protein (24 hour UPr), creatinine clearance rate (Ccr), total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL), high-density lipoprotein (HDL), serum levels of interferon (IFN)-gamma, tumour necrosis factor (TNF)-alpha, interleukin (IL)−4, and IL-10 were measured. Glomerular morphology was observed by light microscopy.ResultsCompared with rats in the control group, rats in the DN group had significantly increased 24 hour UPr, Scr, blood lipids and pro-inflammatory cytokines IFN-gamma and TNF-alpha secreted by Th1 cells. In addition, down-regulation of the proportion of Th2 cells and decrease of the anti-inflammatory cytokines IL-4 and IL-10 secreted by Th2 cells occurred. Compared with the model group, the DSS group had significantly decreased levels of 24 hour UPr, Scr, TC, TG, LDL, IFN-gamma and TNF-alpha, and increased IL-4 and IL-10. The renal pathological changes in the DSS treatment group were ameliorated.ConclusionThis study suggests that DSS could improve renal function in streptozotocin-induced DN model rats. The mechanism may be related to regulating the Th1/Th2 cell balance and improved lipid metabolism in DN rats.AcknowledgmentSupported by a project grant from The National Natural Science Foundation of China (Grant No. 81603527), Science and technology project of Henan Province (Grant No. 162102310466), Key scientific research projects of Henan Province Colleges and Universities (Grant No. 16A360010), Henan University of Traditional Chinese Medicine Scientific and technological innovation talent support program (Grant No. 2015XCXRC05), Science and technology project of Zhengzhou City (Grant No. 20150310).
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