Differential expression of L-FABP and L-BABP between fat and lean chickens

Zhang, Q; Shi, Hui; Liu, W; Wang, Y; Wang, Q; Li, H

doi:10.4238/2013.october.7.5

Cited by 12 publications

(10 citation statements)

References 36 publications

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“…In the present study, L-FABP mRNA levels of CLA-fed chickens were significantly different compared to control (P<0.05). Thus, we suggest that activation of PPAR α induced the expression of L-FABP as PPAR α is the dominant regulator of L-FABP gene in chickens (Zhang et al, 2013).…”

Section: Discussionmentioning

confidence: 73%

“…Murai et al (2009) reported that L-FABP gene involved in hepatic fatty acid metabolism in chicken liver and it is influenced by several factors, including food deprivation, high-fat diets and peroxisome proliferators. L-FABP protein level is itself regulated by PPAR α transcriptional activity in chickens (Zhang et al, 2013). According to Wang et al (2006), L-FABP gene may be associated with fat traits in the chicken.…”

Section: Introductionmentioning

confidence: 99%

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Upregulation of Peroxisome Proliferator-Activated Receptors and Liver Fatty Acid Binding Protein in Hepatic Cells of Broiler Chicken Supplemented with Conjugated Linoleic Acids

Ramiah

Goh

Ebrahimi

2015

Italian Journal of Animal Science

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Since conjugated linoleic acid (CLA) has structural and physiological characteristics similar to peroxisome proliferators, it is hypothesized that CLA would upregulate peroxisome proliferator-activated receptor (PPAR) and liver fatty acid binding protein (L-FABP) in the liver of broiler chicken. The aim of the present study was to determine fatty acid composition of liver in CLA-fed broiler chickens and the genes associated with hepatic lipid metabolism. A total of 180-day-old broiler chicks were randomly assigned to two diets containing 0 and 2.5% CLA and fed for 6 weeks. Fatty acid (FA) composition of liver and PPAR α and γ and L-FABP were analyzed. It has been demonstrated that CLA was found in the liver of CLA-feed chicken compared to control group. Hepatic PPAR α and γ mRNA levels were upregulated 1.2 and 3-fold in CLA-fed chickens compared to chickens fed diet without CLA respectively. A similar response of upregulation was observed for L-FABP mRNA expression. Our data highlights the role of PPARs as a core regulator in the regulation of lipid metabolism in chicken liver.

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Section: Discussionmentioning

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Upregulation of Peroxisome Proliferator-Activated Receptors and Liver Fatty Acid Binding Protein in Hepatic Cells of Broiler Chicken Supplemented with Conjugated Linoleic Acids

Ramiah

Goh

Ebrahimi

2015

Italian Journal of Animal Science

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“…FABP2 has been identified as a specific marker for the relative amount of epithelium in humans and pigs ( 96 ). Several FABP (FABP1, FABP2, FABP6, and FABP10) have been identified to be predominantly expressed in the digestive tract of chickens ( 97 , 98 ); however, much remains to be determined regarding their expression and biological functions in poultry. FABP10 plays an important hepatic role in in response to FI in chicken ( 98 ).…”

Section: Discussionmentioning

confidence: 99%

Identification of Potential Biomarkers for Gut Barrier Failure in Broiler Chickens

et al. 2015

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The objective of the present study was to identify potential biomarkers for gut barrier failure in chickens. A total of 144 day-of-hatch Ross 308 male broiler chickens were housed in 24 battery cages with six chicks per cage. Cages were randomly assigned to either a control group (CON) or gut barrier failure (GBF) group. During the first 13 days, birds in CON or GBF groups were fed a common corn–soy starter diet. On day 14, CON chickens were switched to a corn grower diet, and GBF chickens were switched to rye–wheat–barley grower diet. In addition, on day 21, GBF chickens were orally challenged with a coccidiosis vaccine. At days 21 and 28, birds were weighed by cage and feed intake was recorded to calculate feed conversion ratio. At day 28, one chicken from each cage was euthanized to collect intestinal samples for morphometric analysis, blood for serum, and intestinal mucosa scrapings for gene expression. Overall performance and feed efficiency was severely affected (P < 0.05) by a GBF model when compared with CON group at days 21 and 28. Duodenum of GBF birds had wider villi, longer crypt depth, and higher crypt depth/villi height ratio than CON birds. Similarly, GBF birds had longer crypt depth in jejunum and ileum when compared with CON birds. Protein levels of endotoxin and α1-acid glycoprotein (AGP) in serum, as well as mRNA levels of interleukin (IL)-8, IL-1β, transforming growth factor (TGF)-β4, and fatty acid-binding protein (FABP) 6 were increased (P < 0.05) in GBF birds compared to CON birds; however, mRNA levels of FABP2, occludin, and mucin 2 (MUC2) were reduced by 34% (P < 0.05), 24% (P = 0.107), and 29% (P = 0.088), respectively, in GBF birds compared to CON birds. The results from the present study suggest that serum endotoxin and AGP, as well as, gene expression of FABP2, FABP6, IL-8, IL-1β, TGF-β4, occludin, and MUC2 in mucosa may work as potential biomarkers for gut barrier health in chickens.

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“…However, FABP4 may regulate abdominal fat deposition through lipolysis. Another two types of fatty acid-binding protein subfamily named L-FABP (liver fatty acid-binding protein) and liver “basic” fatty acid-binding protein (Lb-FABP) have been investigated their expression in liver between fat and lean male-chickens by Zhang et al ( 2013 ). L-FABP mRNA expression in fat birds was higher than that in lean birds at 2, 3, 4, 5, 6, and 10 weeks of age, and L-BABP mRNA expression in fat birds was higher than that in lean birds at 1, 2, 3, 4, 8, and 10 wk of age.…”

Section: Introductionmentioning

confidence: 99%

Genomic Insights Into the Multiple Factors Controlling Abdominal Fat Deposition in a Chicken Model

Abdalla

Chen²,

Nie³

et al. 2018

Front. Genet.

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Genetic selection for an increased growth rate in meat-type chickens has been accompanied by excessive fat accumulation particularly in abdominal cavity. These progressed to indirect and often unhealthy effects on meat quality properties and increased feed cost. Advances in genomics technology over recent years have led to the surprising discoveries that the genome is more complex than previously thought. Studies have identified multiple-genetic factors associated with abdominal fat deposition. Meanwhile, the obesity epidemic has focused attention on adipose tissue and the development of adipocytes. The aim of this review is to summarize the current understanding of genetic/epigenetic factors associated with abdominal fat deposition, or as it relates to the proliferation and differentiation of preadipocytes in chicken. The results discussed here have been identified by different genomic approaches, such as QTL-based studies, the candidate gene approach, epistatic interaction, copy number variation, single-nucleotide polymorphism screening, selection signature analysis, genome-wide association studies, RNA sequencing, and bisulfite sequencing. The studies mentioned in this review have described multiple-genetic factors involved in an abdominal fat deposition. Therefore, it is inevitable to further study the multiple-genetic factors in-depth to develop novel molecular markers or potential targets, which will provide promising applications for reducing abdominal fat deposition in meat-type chicken.

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Differential expression of L-FABP and L-BABP between fat and lean chickens

Cited by 12 publications

References 36 publications

Upregulation of Peroxisome Proliferator-Activated Receptors and Liver Fatty Acid Binding Protein in Hepatic Cells of Broiler Chicken Supplemented with Conjugated Linoleic Acids

Upregulation of Peroxisome Proliferator-Activated Receptors and Liver Fatty Acid Binding Protein in Hepatic Cells of Broiler Chicken Supplemented with Conjugated Linoleic Acids

Identification of Potential Biomarkers for Gut Barrier Failure in Broiler Chickens

Genomic Insights Into the Multiple Factors Controlling Abdominal Fat Deposition in a Chicken Model

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