A total of 389 microRNA (miRNA) were identified in small extracellular vesicles (sEV) isolated from bovine colostrum, with the top 50 miRNA contributing to more than 90% of total abundance and predicted to target 2,655 genes associated with cellular processes, environmental information processing, and organismal systems. The expression profiles of sEV-associated miRNA in bovine colostrum was independent of the concentrations of immunoglobulin G.
Highlights• The RNA concentration in sEV isolated from bovine colostrum was highest using the combination of the miRCURY Cell/Urine/CSF and miRNeasy Mini kits. • The top 50 miRNA were the same using miRDeep2 and sRNAbench, predominated by let-7b, let-7a-5p, miR-30a-5p, and miR-148a. • Predicted target genes of the top 50 miRNA regulate PI3K-Akt and MAPK signaling pathways, axon guidance, and focal adhesion. • The abundance of miR-27a-3p was higher in colostrum with high IgG concentrations.
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