Assessment of microRNA profiles in small extracellular vesicles isolated from bovine colostrum with different immunoglobulin G concentrations

Ma, Tao; Li, W.; Chen, Y.; Cobo, Eduardo R.; Windeyer, Claire; Gamsjäger, Lisa; Diao, Qiyu; Tu, YuShan; Guan, Le Luo

doi:10.3168/jdsc.2022-0225

Cited by 6 publications

(5 citation statements)

References 48 publications

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“…Some miRNAs have a higher expression level in BC than in milk, such as miR-142-5p, miR-150, miR-155, miR-181a, and miR-223 [163,345]. Ma et al [353] found that colostrum with very different IgG levels (62.8 ± 3.6 and 256.5 ± 5.7 mg/mL) had a similar expression profile of miRNAs present in small EVs, which contributes to the statement that colostrum with a higher concentration of IgG does not necessarily indicate higher concentrations of the many other BC reviewed in the present article. Nevertheless, studies correlating IgG and other BC are still lacking.…”

Section: Growth Factorsmentioning

confidence: 99%

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A Comprehensive Review of the Variability of Bovine Colostrum Components and Their Effect on Neonatal Calf Physiology

Da Silva,

Silva,

Pereira

et al. 2024

Preprint

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Most research on colostrum quality and colostrum management focuses on maternal IgG transfer to the newborn calf. However, in addition to being a source of antibodies, colostrum has other components that influence passive immune transfer, nutrition, gut maturation, and thermoregulation. In this review, the constituents in the bovine colostrum, as well as their variation, are explored and it is discussed the biological role of colostrum on the newborn calf. Protein is the nutrient in higher concentration and fat is the nutrient with the most variation. There is a pronounced amplitude in the total solids content of colostrum, but the average concentration does not vary much between studies (24.7%). Bovine colostrum major components can vary with several factors, such as the region, season, breed, dry period length, and nutritional aspects of the dam. Bioactive components, cells, and microbiome variability are less understood, besides IgG. Regardless, they play crucial distinct roles in the physiological development of the newborn. However, a multi-interactive effect between these components, cells, and microorganisms is more likely to occur. Recently researched components in colostrum like miRNA, nucleo(t)sides, the milk fat globule membrane, oligosaccharides, minor proteins, and the microbiome are examples of promising interest areas of study.

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Section: Growth Factorsmentioning

confidence: 99%

“…Nevertheless, studies correlating IgG and other BC are still lacking. Only one miRNA was more abundant in high-IgG colostrum-miR-27a-3p-which is associated with osteogenic differentiation in pre-osteoblasts [353] and with glycaemic and lipid status in women with gestational diabetes mellitus [354].…”

Section: Growth Factorsmentioning

confidence: 99%

A Comprehensive Review of the Variability of Bovine Colostrum Components and Their Effect on Neonatal Calf Physiology

Da Silva,

Silva,

Pereira

et al. 2024

Preprint

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“…A bovine transcriptomic analysis showed mEV-enriched miRNAs were associated with milk synthesis and milk fat and protein metabolism compared to colosEVs; in contrast, immune-system-related miRNAs, which showed many differences between the two breeds under study, were particularly found in colosEVs, making the breed a variable to be taken into account for mEV characterisation and application [ 194 ]. Surprisingly, no differences in miRNA cargo were observed in cow colosEVs derived from high- and low-immunoglobulin colostrum with the unique exception for miR-27a-3p, which was associated with high-IgG; this indicated a core of miRNAs essential for the health and developmental stages [ 195 ]. The differences that characterise EVs in milk during lactation periods are reflected in vesicle functionalities.…”

Section: Intrinsic Therapeutic Potential Of Mevsmentioning

confidence: 99%

Extracellular Vesicles from Animal Milk: Great Potentialities and Critical Issues

Mecocci

Trabalza‐Marinucci

Cappelli

2022

Animals

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Other than representing the main source of nutrition for newborn mammals, milk delivers a sophisticated signaling system from mother to child that promotes postnatal health. The bioactive components transferred through the milk intake are important for the development of the newborn immune system and include oligosaccharides, lactoferrin, lysozyme, α-La, and immunoglobulins. In the last 15 years, a pivotal role in this mother-to-child exchange has been attributed to extracellular vesicles (EVs). EVs are micro- and nanosized structures enclosed in a phospholipidic double-layer membrane that are produced by all cell types and released in the extracellular environment, reaching both close and distant cells. EVs mediate the intercellular cross-talk from the producing to the receiving cell through the transfer of molecules contained within them such as proteins, antigens, lipids, metabolites, RNAs, and DNA fragments. The complex cargo can induce a wide range of functional modulations in the recipient cell (i.e., anti-inflammatory, immunomodulating, angiogenetic, and pro-regenerative modulations) depending on the type of producing cells and the stimuli that these cells receive. EVs can be recovered from every biological fluid, including blood, urine, bronchoalveolar lavage fluid, saliva, bile, and milk, which is one of the most promising scalable vesicle sources. This review aimed to present the state-of-the-art of animal-milk-derived EV (mEV) studies due to the exponential growth of this field. A focus on the beneficial potentialities for human health and the issues of studying vesicles from milk, particularly for the analytical methodologies applied, is reported.

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“…Bovine colostrum is also abundant in lactoferrin ( Stelwagen et al., 2009 ), lactoperoxidase, caseins, cathepsins, oligosaccharides ( Arslan et al., 2021 ), and cathelicidins ( Stelwagen et al., 2009 ; Isobe et al., 2013 ), which promote gut integrity in pigs ( Otte et al., 2009 ; Yi et al., 2016 ). Other non-IgG biomolecules are non-coding RNAs and microRNAs (miRNAs) ( Lau et al., 2001 ), present in the bovine mammary gland ( Putz et al., 2019 ; Sun et al., 2019 ) and milk ( Chen et al., 2010 ) in association with IgG ( Ma et al., 2022 ), which are capable of epigenetically regulating gene expression of the host ( Pritchard et al., 2012 ). However, research on bovine colostrum has been mostly focused on ensuring transfer of passive immunity and gut development in newborn calves, while its effect as a treatment at the onset of diarrheic cryptosporidiosis is unknown.…”

Section: Introductionmentioning

confidence: 99%

Host innate immune responses and microbiome profile of neonatal calves challenged with Cryptosporidium parvum and the effect of bovine colostrum supplementation

Gamsjäger

Cirone

Schluessel

et al. 2023

Front. Cell. Infect. Microbiol.

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IntroductionCalves are highly susceptible to gastrointestinal infection with Cryptosporidium parvum (C. parvum), which can result in watery diarrhea and eventually death or impaired development. With little to no effective therapeutics, understanding the host’s microbiota and pathogen interaction at the mucosal immune system has been critical to identify and test novel control strategies.MethodsHerein, we used an experimental model of C. parvum challenge in neonatal calves to describe the clinical signs and histological and proteomic profiling of the mucosal innate immunity and microbiota shifts by metagenomics in the ileum and colon during cryptosporidiosis. Also, we investigated the impact of supplemental colostrum feeding on C. parvum infection.ResultsWe showed that C. parvum challenged calves experienced clinical signs including pyrexia and diarrhea 5 days post challenge. These calves showed ulcerative neutrophil ileitis with a proteomic signature driven by inflammatory effectors, including reactive oxygen species and myeloperoxidases. Colitis was also noticed with an aggravated mucin barrier depletion and incompletely filled goblet cells. The C. parvum challenged calves also displayed a pronounced dysbiosis with a high prevalence of Clostridium species (spp.) and number of exotoxins, adherence factors, and secretion systems related to Clostridium spp. and other enteropathogens, including Campylobacter spp., Escherichia sp., Shigella spp., and Listeria spp. Daily supplementation with a high-quality bovine colostrum product mitigated some of the clinical signs and modulated the gut immune response and concomitant microbiota to a pattern more similar to that of healthy unchallenged calves.DiscussionC. parvum infection in neonatal calves provoked severe diarrheic neutrophilic enterocolitis, perhaps augmented due to the lack of fully developed innate gut defenses. Colostrum supplementation showed limited effect mitigating diarrhea but demonstrated some clinical alleviation and specific modulatory influence on host gut immune responses and concomitant microbiota.

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Assessment of microRNA profiles in small extracellular vesicles isolated from bovine colostrum with different immunoglobulin G concentrations

Cited by 6 publications

References 48 publications

A Comprehensive Review of the Variability of Bovine Colostrum Components and Their Effect on Neonatal Calf Physiology

A Comprehensive Review of the Variability of Bovine Colostrum Components and Their Effect on Neonatal Calf Physiology

Extracellular Vesicles from Animal Milk: Great Potentialities and Critical Issues

Host innate immune responses and microbiome profile of neonatal calves challenged with Cryptosporidium parvum and the effect of bovine colostrum supplementation

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