Our results might be helpful for explaining the missing heritability of the psoriasis due to epistasis and provide a deep insight into the important role of the IL23/Th17 pathway in the pathogenesis of psoriasis.
Cold stress restricts plant development seriously, resulting in heavy
agricultural losses. We found a critical transcription factor network in
Medicago ruthenica is involved in plant adaptation to low
temperature. APETALA2/ETHYLENE RESPONSIVE FACTOR (AP2/ERF) transcription
factor MrERF039 was transcriptionally induced by cold stresses in
M. ruthenica. We established an agrobacterium-mediated transient
expression system and found that MrERF039 plays an active role in
the cold response of M. ruthenica. Overexpression of
MrERF039 significantly increased the soluble sugar content, which
was eventually expressed as the accumulation of glucose and maltose.
Electrophoretic mobility shift assays (EMSA) and yeast monohybrid
crosses showed that MrERF039 could bind to the DRE cis-acting element on
the MrCAS15A promoter. Additionally, the methyl group of the 14th
amino acid in the MrERF039 protein was required for the binding.
Transcriptome results analysis showed that the MrERF039 gene
acted as a sugar molecular switch, regulating many sugar transporters
and sugar metabolism related genes. In addition, we found that
MrERF039 can directly regulate the expression of β-amylase gene,
UDP glycosyltransferase gene and C2H2 zinc finger protein gene. And the
significant increase in glucose and maltose content may be caused by the
breakdown of starch degraded by β-amylase. In conclusion, these findings
suggest that high expression of the MrERF039 gene can
significantly improve the cold tolerance of M. ruthenica root
tissues during cold acclimation. These provides a new theoretical basis
and candidate genes for breeding new legume forage varieties with high
resistance, high yield and high protein.
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