A modification of the method of Burrows & Quinn (1939) was successfully used to collect semen from male Japanese quail, and females were artificially inseminated by intravaginal, intraperitoneal and intrauterine routes. Others were mated naturally. Semen from antibiotic-fed males, diluted with quail-egg albumin containing antibiotics, and deposited in the uterus (shell gland) by means of a hypodermic needle passed through the egg contained therein, fertilized more than 75 % of the females for a mean duration of 4\m=.\6 days. This procedure also resulted in excellent egg production and caused no mortality. In contrast, the other methods of artificial insemination resulted in much lower fertility and egg production and, in some cases, heavy mortality.
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