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BackgroundLeptin and its receptor play a role in several disease processes such as pancreatitis and heart disease. However, their association with gallbladder mucocele (GBM) in dogs has not been reported.Hypothesis/ObjectivesTo evaluate differences in the expression of leptin and leptin receptor between dogs with and without GBM.AnimalsTwenty‐five healthy dogs, including 9 laboratory beagle dogs, and 22 client‐owned dogs with GBM.MethodsSerum leptin concentration was determined in blood samples of all dogs by ELISA. Canine gallbladder samples were collected from 9 dogs with GBM that underwent surgery for therapeutic purposes and from 9 healthy laboratory beagle dogs as a normal control group. Samples were analyzed for leptin and leptin receptor mRNA by real‐time polymerase chain reaction.ResultsSerum leptin concentration was significantly higher in dogs with GBM than in healthy dogs (medians of 7.03 and 2.18 ng/mL, respectively; P < .001). Patients with GBM that had undergone surgery had significantly higher serum leptin concentrations than those that had not (medians of 12.2 and 4.09 ng/mL, respectively; P = .001). However, no difference in serum leptin concentration was found between dogs with GBM with or without endocrinopathies. The mRNA expression levels of leptin and its receptor were significantly increased in the gallbladder tissues of dogs with GBM.Conclusions and Clinical ImportanceDysregulation of leptin might be involved in the pathophysiology of GBM, and leptin concentrations might be associated with GBM severity.

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level of heterogeneity that included the same tumour subtypes also obtained using the ex vivo procedure. Conclusion A set of the same genetic mutations is able to drive heterogeneous MM development suggesting that the specific phenotype of the resulting tumour depends on the specific cellof-origin. Therefore, epigenetic differences rather than acquired mutations appear a determining factor for the subtype of MM that arises. This epigenetic state appears relative stability as interconversion of the tumour subtypes was not observed. PO-275EFFECT Introduction Head and neck cancer includes malignancies usually originated in upper aerodigestive tract mucosa. Despite hypopharyngeal cancer has a low incidence, patients have poor prognosis, advanced stage and distant metastization. A low overall survival of these cancer might be explained by the presence of cancer stem cells (CSC). Identification of a CSCs markers panel could help to overcome this barrier allowing to develop target drugs. This study aims to evaluate and characterise the expression of proliferation, adhesion and CSC markers in a hypopharyngeal cell line (FaDu) before and after ionising radiation exposure. Material and methods Paraffin cell blocks were performed in order to evaluate by immunohistochemistry (IHC), epithelial characterisation (cytokeratin CAM 5.2, leukocyte common antigen (LCA), vimentin), tumoral aggressiveness (P16, P53, OCT4 and SALL4), proliferation (Ki-67) and CSC markers (EpCAM, CD10, CD44, CD117, CD133, beta-catenin). Also, 14 subtypes of high-risk HPV (31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68, 16, and 18), EBV, Akt and Wnt expressions were analysed. Radiotherapy was performed with 0.5 × 10 6 cells/mL, exposed to increasing doses of X-ray (0.5 to 10 Gy), except for control cells. Clonogenic assay and IHC were realised. Treated cells for IHC were fixed with alcohol 96%, 48 hour after treatment and posteriorly analysed the following antigens EpCAM, CD133, CD44, CD117, CD10, Akt, Wnt, OCT4 and P53. Results and discussions FaDu cell line showed positive immunostaining for cytokeratin, vimentin and negative to LCA. Relatively to tumoral aggressiveness, cells only expressed positively P16, while P53, OCT4 and SALL4 were negative. About proliferation it was observed Ki-67+. Between analysed stemness markers just CD44, CD133 and beta-catenin were expressed. Among HPV subtypes analysed only HPV18 was stained positive and EBV-. Adding, control cells expressed Akt +and Wnt +in normal conditions. Preliminary results related to ionising radiation effects showed that cell survival is X-ray dose dependent. Moreover, it was observed Wnt expression alterations with X-ray exposure.