A total of 100 random clinical urine specimens were divided and used to compare Becton, Dickinson & Co. B-D Urine Culture Kit-treated urine (BDU) with refrigerated urine (RU). Each RU-BDU pair was cultured with the standard calibrated loop-plate method and also screened with the Abbott Laboratories MS-2 system at 0, 5, and 24 h. An additional 158 positive urine specimens containing >105 CFU/ml were tested by standard culturing and MS-2 at 0 and 24 h. In addition, growth curves were run for Escherichia coli containing RU-BDU pairs at 0 and 24 h, and MS-2 detection time frequencies were analyzed. Culture results showed few changes in colony counts at 0 and 5 h for RU and BDU specimens. At 24 h, there were decreased colony counts for 7.0% of RU and 17.7% of BDU specimens. MS-2 results showed detection rates at 0 h of 100% for RU and 82.9o for BDU specimens and at 24 h of 91.1% for RU and 63.3% for BDU specimens. The organisms most frequently missed at 24 h were E. coli, Pseudomonas aeruginosa, and Staphylococcus epidermidis. Growth curve analysis showed a 1-h delay of logarithmic growth in BDU specimens. MS-2 detection time analysis showed delayed positive reports owing to the extended detection time for BDU specimens. The B-D Urine Culture Kit is a convenient transport system; however, some difficulty in recovering organisms at 24 h with the standard culture method and increased difficulty with the MS-2 system were recognized.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.