For optimization of biochemical processes in food and pharmaceutical industries, the evaluation of enzyme inactivation kinetic models is necessary to allow their adequate use. Kinetic studies of thermal inactivation of βgalactosidase from Aspergillus oryzae were conducted in order to critically evaluate mathematical equations presented in the literature. Statistical analysis showed that Weibull model presented the best adequacy to residual enzymatic activity data through the processing time and its kinetic parameters as a function of the temperature, in the range of 58-66 ºC. The investigation suggests the existence of a non-sensitive heat fraction on the enzyme structure, which is relatively stable up to temperatures close to 59 ºC. Thermodynamic parameters were evaluated and showed that such β-galactosidase presents activation energy of 277 kJ mol-1 and that the enzyme inactivation is due to molecular structural changes. Results shown that the enzyme is quite stable for biotechnological applications.
____________________________________________________________________________________________________________ RESUMOO bagaço de cana-de-açúcar é um resíduo sólido amplamente produzido no Brasil e representa uma ótima oportunidade para explorá-lo como fonte de compostos funcionais. Assim, o objetivo do presente trabalho é avaliar a concentração de polifenóis totais, compostos com atividade antioxidante e antibacteriana. Os resultados mostraram que o extrato aquoso do bagaço de cana não foi capaz de inibir o crescimento de importantes bactérias de origem alimentar. O conteúdo total de polifenóis foi de 728.201±58,21 mg de ácido gálico equivalente por 100g e flavonóis de 325.143 ± 19,03 mg de equivalente de rutina por 100 gramas de bagaço seco. A capacidade antioxidante foi capaz de sequestrar 5,05%±0,003% dos radicais DPPH e apresentou capacidade de sequestro de ABTS de 67.535 ± 10,44 mmol de equivalente de Trolox por 100 gramas de bagaço seco. Assim, o presente trabalho pode contribuir para futuros estudos para avaliar o resíduo a ser utilizado como componente funcional em aplicações industriais.
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