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BACKGROUND: Ischemia reperfusion causes injury to the liver cells during transplantation, trauma and emergency surgery. We investigated whether the anti TNF-α agent, etanercept, can reduce injury in an animal model of ischemia reperfusion owing to the fact that TNF-α plays a critical role in the process of infl ammation. MATERIALS AND METHODS: Thirty rats were divided into three groups: sham (Group 1), control (Group 2), etanercept (5 mg/kg) treatment (Group 3). Ischemia-reperfusion model was carried out by clamping the hepatic pedicle for 45 min and then reperfusing the liver for 60 min. Etanercept (5 mg/kg) was injected intraperitoneally 5 min prior to reperfusion. At the end of the procedures, blood and liver tissue samples were obtained for biochemical and histopathological assessment. RESULTS: Control and treatment groups showed signifi cant differences in hepatic function tests, plasma and tissue oxidative stress parameters. Samples in the control group histopathologically showed morphologic abnormalities specifi c to ischemia reperfusion. Histomorphologic fi ndings in the treatment groups showed similar features as the sham group. CONCLUSIONS: Our evidence suggests that TNF-α plays a key role in liver ischemia reperfusion injury and etanercept may provide a novel therapeutic approach for patients undergoing liver surgical procedure (Tab. 3, Fig. 4, Ref. 22). Text in PDF www.elis.sk. A, Offi dani A. The effect of etanercept on hepatic fi brosis risk in patients with non-alcoholic fatty liver disease, metabolic syndrome, and psoriasis. J Gastroenterol 2012 (10). Tian Y, Jochum W, Georgiev P, Moritz W, Graf R, Clavien PA.Kupffer cell-dependent TNF-alpha signaling mediates injury in the arterialized small-for-size liver transplantation in the mouse.

Purpose: To examine the effect of taxifolin on I/R induced gastric injury in rats using biochemical and histopatholohical methods. Methods: Eighteen albino Wistar male rats equally grouped as; gastric I/R (I/R), 50 mg/kg taxifolin + gastric I/R (TAX+ I/R) and sham operation applied (SHAM). Ischemia induced for 1 hour, and reperfusion induced for 3 hours. Results: Oxidant parameters like, Malondialdehyde (MDA) and Hydroxyguanine (8-OHdG) were higher, whereas total glutathione (tGSH) was lower in the I/R group according to SHAM group, histopathological findings such as marked destruction, edema, and proliferated dilated congested blood vessels were observed severely in the I/R group, whereas there was not any pathological finding except mild dilated congested blood vessels in the TAX+ I/R group. Conclusion: The taxifolin can be clinically beneficial in the treatment of gastric injury due to I/R procedure.

Background: Hepatic ischemia-reperfusion causes histological injury to the pancreatic cells during transplantation, trauma and emergency surgery. We investigated the effects of diosmin, a phlebotrophic drug with antioxidant and antiinfl ammatory effects, on pancreatic injury in the experimental liver ischemia-reperfusion model. Materials and methods: Forty rats were divided into the four groups: sham (Group 1), control (Group 2), peroperative diosmin (50 mg/kg) treatment (Group 3) and preoperative 10-day diosmin (50 mg/kg) treatment group (Group 4). Ischemia-reperfusion model was carried out by clamping the hepatic pedicle for 60 min and then reperfusing the liver for 90 min. At the end of the procedures, blood and pancreas tissue samples were obtained for biochemical and histopathological assessment. Results: According to the results of liver function tests, amylase and the plasma and pancreatic tissue oxidative stress parameters, there was a signifi cant difference between the control and other groups. Histopathologically; the specimens of the Group 2 showed specifi c morphological abnormalities. The groups 3 and 4 showed the pancreas histomorphology similar to the sham group. Pathological scores were signifi cantly different between the Group 2 and other groups. Conclusions: Diosmin can be administered for a protection of destructive effects of hepatic ischemia-reperfusion injury on pancreas both emergent and elective hepatic surgical operations in which possible ischemic periods were expected (Fig.

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