This study deals with the effects of hydrodynamic functioning of hard-rock aquifers on microbial communities. In hard-rock aquifers, the heterogeneous hydrologic circulation strongly constrains groundwater residence time, hydrochemistry, and nutrient supply. Here, residence time and a wide range of environmental factors were used to test the influence of groundwater circulation on active microbial community composition, assessed by high throughput sequencing of 16S rRNA. Groundwater of different ages was sampled along hydrogeologic paths or loops, in three contrasting hard-rock aquifers in Brittany (France). Microbial community composition was driven by groundwater residence time and hydrogeologic loop position. In recent groundwater, in the upper section of the aquifers or in their recharge zone, surface water inputs caused high nitrate concentration and the predominance of putative denitrifiers. Although denitrification does not seem to fully decrease nitrate concentrations due to low dissolved organic carbon concentrations, nitrate input has a major effect on microbial communities. The occurrence of taxa possibly associated with the application of organic fertilizers was also noticed. In ancient isolated groundwater, an ecosystem based on Fe(II)/Fe(III) and S/SO4 redox cycling was observed down to several 100 of meters below the surface. In this depth section, microbial communities were dominated by iron oxidizing bacteria belonging to Gallionellaceae. The latter were associated to old groundwater with high Fe concentrations mixed to a small but not null percentage of recent groundwater inducing oxygen concentrations below 2.5 mg/L. These two types of microbial community were observed in the three sites, independently of site geology and aquifer geometry, indicating hydrogeologic circulation exercises a major control on microbial communities.

International audienceCrystalline-rock aquifers generally yield limited groundwater resources. However, some highly productive aquifers may be encountered, typically near tectonic discontinuities. In this study, we used a multidisciplinary experimental field approach to investigate the hydrogeological behavior of a sub-vertical permeable fault zone identified by lineament mapping. We particularly focused our investigations on the hydrogeological interactions with neighboring reservoirs. The geometry of the permeable domains was identified from geological information and hydraulic test interpretations. The system was characterized under natural conditions and during a 9-week large-scale pumping test. We used a combination of piezometric analysis, flow logs, groundwater dating and tracer tests to describe the interactions between permeable domains and the general hydrodynamical behaviors. A clear vertical compartmentalization and a strong spatial heterogeneity of permeability are highlighted. Under ambient conditions, the vertical permeable fault zone allows discharge of deep groundwater flows within the superficial permeable domain. The estimated flow across the total length of the fault zone ranged from 170 to 200 m3/day. Under pumping conditions, hydrological data and groundwater dating clearly indicated a flow inversion. The fault zone appears to be highly dependent on the surrounding reservoirs which mainly ensure its recharge. Groundwater fluxes were estimated from tracer tests interpretation. This study demonstrates the hydrogeological capacities of a sub-vertical fault aquifer in a crystalline context. By describing the hydrological behavior of a fault zone, this study provides important constrain about groundwater management and protection of such resources