BackgroundDespite the implementation of prevention guidelines, early-onset group B streptococci (GBS) disease remains a cause of neonatal morbidity and mortality worldwide. Strategies to identify women who are at risk of transmitting GBS to their infant and the administration of intrapartum antibiotics have greatly reduced the incidence of neonatal GBS disease. However, there is a requirement for a rapid diagnostic test for GBS that can be carried out in a labour ward setting especially for women whose GBS colonisation status is unknown at the time of delivery. We report the design and evaluation of a real-time PCR test (RiboSEQ GBS test) for the identification of GBS in vaginal swabs from pregnant women.MethodsThe qualitative real-time PCR RiboSEQ GBS test was designed based on the bacterial ssrA gene and incorporates a competitive internal standard control. The analytical sensitivity of the test was established using crude lysate extracted from serial dilutions of overnight GBS culture using the IDI Lysis kit. Specificity studies were performed using DNA prepared from a panel of GBS strains, related streptococci and other species found in the genital tract environment. The RiboSEQ GBS test was evaluated on 159 vaginal swabs from pregnant women and compared with the GeneOhm™ StrepB Assay and culture for the identification of GBS.ResultsThe RiboSEQ GBS test is specific and has an analytical sensitivity of 1-10 cell equivalents. The RiboSEQ GBS test was 96.4% sensitive and 95.8% specific compared to "gold standard" culture for the identification of GBS in vaginal swabs from pregnant women. In this study, the RiboSEQ GBS test performed slightly better than the commercial BD GeneOhm™ StrepB Assay which gave a sensitivity of 94.6% and a specificity of 89.6% compared to culture.ConclusionThe RiboSEQ GBS test is a valuable method for the rapid, sensitive and specific detection of GBS in pregnant women. This study also validates the ssrA gene as a suitable and versatile target for nucleic acid-based diagnostic tests for bacterial pathogens.

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This paper presents the findings of an expert working group of researchers from East Asian countries. The group was tasked with developing a theoretically sound and practically implementable methodology for assessing the sustainability of biomass utilisation in East Asian countries based on the needs and potential of biomass resources in this region. Building on six years of research conducted between 2007 and 2013, the working group formulated a set of main and secondary indicators for biomass utilisation under three pillars of sustainability. For the environmental pillar, the main indicator was life cycle greenhouse gas emissions and secondary indicators were water consumption and soil quality. For the economic pillar, the main indicator was total value added and secondary indicators were net profit, productivity, and net energy balance. For the social pillar, the main indicators were employment generation and access to modern energy, and the secondary indicator was the human development index. The application of the working group methodology and indicators in sustainability assessments of biomass utilisation will enable decision makers in East Asian countries to compare the sustainability of biomass utilisation options and to make decisions on whether or not to launch or sustain biomass utilisation initiatives.

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