ResumoA bioquímica sérica permite estabelecer valores de referência utilizados na avaliação clínica e metabólica de bezerros, reduzindo as perdas na criação bovina. Neste contexto, objetivou-se avaliar o perfil bioquímico sérico e a influência do fator etário em bezerros da raça Senepol, do nascimento até 120 dias de idade. O experimento foi realizado no município de Uberlândia-MG, Brasil, em quatro momentos após o nascimento (0 -15º, 30º, 60º e 120º dia de vida). As amostras de sangue foram obtidas por venopunção da jugular para determinação das concentrações séricas de proteínas totais, albumina, globulina, triglicerídeos, colesterol, AST, GGT, FA, cálcio, fósforo, magnésio, creatinina e uréia. O colesterol manteve-se abaixo ou nos valores mínimos de referência em todos os momentos. Os animais aos 30 dias apresentaram os valores mais baixos do proteinograma, minerais e triglicerídeos que se normalizaram aos 60 dias de vida, exceto a albumina que apresentou recuperação mais lenta. Concluiuse que há influência da faixa etária no comportamento dos constituintes bioquímicos analisados e que há diferenças quando comparados a outras raças. Palavras-chave: Bezerros, bioquímica sérica, colesterol, Bos taurus abstract The serum biochemical values allow to establish reference used in metabolic and clinical evaluations of calves, reducing losses in cattle breeding. The aim of this study was to evaluate the influence of age on serum biochemical profile in Senepol calves, at the first 120 days of age. The experiment was conducted in the city of Uberlândia, Minas Gerais, Brazil, in four periods after birth (0-15, 30, 60 and 120 days of age). Blood samples were obtained by jugular venipuncture for determination of serum total protein, albumin, globulin, triglycerides, cholesterol, AST, GGT, FA, calcium, phosphorus, magnesium, creatinine and urea. Cholesterol remained at minimum reference values or below throughout the experiment. Animals at 30 days showed the lowest values of protein concentrations, minerals and triglycerides that normalized up to 60 days of life, except to albumin that slower recovery was showed. Our data showed that the serum biochemical profile in Senepol is influenced by age up to 120 days of age; moreover there are differences with other breeds.

This study evaluated the occurrence of Leishmania spp. in equids from Uberlândia, Minas Gerais, Southeastern Brazil. Serum samples from 257 healthy equids of Uberlândia were assessed for the presence of antibodies against Leishmania spp. by using the indirect fluorescent-antibody test. Additionally, an epidemiological survey was done to evaluate the possible risk factors (species of equids, age, gender, economic activity, and contact with domestic and wild animals) associated with the presence of antibodies against Leishmania spp. The total seroprevalence against Leishmania spp. was 24.1% (62/257); representing seroprevalence levels of 22.7% (55/242) in horses, 50% (4/8) in mules, and 42.9% (3/7) in ponies from all regions evaluated. The species of equid was the only factor that contributed to an elevated seroprevalence of Leishmania spp.

Neospora caninum causes reproductive disorders in dairy cattle such as repeat breeding, abortion, and anestrus, which are risk factors associated with the seroprevalence of N. caninum in Brazil and worldwide. This study aimed to verify the risk factors related to the seroprevalence of Neospora caninum in crossbred dairy cows in the city of Uberlândia, Minas Gerais. During, January to July 2013, 740 blood samples were collected from 57 herds with a history of reproductive disorders and determinations of antibody titers against N. caninum were obtained by ELISA assay. The seroprevalence of N. caninum was 18.4% (136/740). Risk factors for N. caninum were cows greater than 2 years of age (P = 0.034), mechanical milking (P = 0.012), and frequent purchase of animals (P = 0.004), in addition to individual history of abortion (P = 0.007) and repeat breeding (P = 0.030). The highest probability of occurrence of associated risk factors was associated with individual history of abortion and repeated breeding and the frequent purchase of animals, with odds of 12.8, 5.9, and 3.8 times, respectively. In conclusion, the risk factors associated with N. caninum: members of the female sex and cows with more than 2 yearsof-age, mechanical milking, and the frequent purchase of animals, in addition to individual history of abortion and repeated breeding.

ResumoA eficiência reprodutiva é um dos principais fatores envolvidos na viabilidade econômica do setor de criação de bovinos. Objetivou-se realizar a avaliação ginecológica e citológica em vacas Holandesas com mais de três repetições de cio. O experimento foi desenvolvido em seis propriedades leiteiras comerciais, com manejos sanitários e reprodutivos semelhantes e assistidas por médicos veterinários. Foram incluídas apenas vacas da raça Holandesa, até cinco parições e mais de três repetições de cio consecutivas. Na propriedade, os animais foram identificados e procedidos o exame ginecológico e citologia endometrial. No presente estudo foram avaliadas 77 vacas e as causas reprodutivas encontradas em vacas Holandesas sujeitas ao descarte involuntário foram: endometrite clínica com secreção vaginal purulenta e mais que 3% de neutrófilos (7,79%), endometrite clínica com secreção vaginal purulenta e menos que 3% de neutrófilos (2,6%), endometrite subclínica (10,4%), urovagina (1,3%) e 77,9% que não se sabe a causa, porém não está associado aos distúrbios reprodutivos avaliados. Concluiu-se que: a) o índice de vacas que não apresentaram a causa diagnosticada é muito alto e isto interfere diretamente na eficiência reprodutiva; b) o exame proposto diagnosticou 22,1% de distúrbios clínicos que poderiam ser evitados ou tratados e desta forma reduzindo o descarte involuntário. Palavras-chave: Bovinos, endometrite clínica, endometrite subclínica, urovagina AbstractThe objective of this study was to evaluate the gynecological examination and endometrial cytology in Holstein cows with more than three repetitions of estrus. The experiment was conducted in six commercial dairy herds both with similar reproductive and sanitary managements and assisted by veterinarians. It was included only Holstein cows up to five calving and more than three consecutive repetitions of estrus. Seventy-seven cows were identified and carried gynecological examination and endometrial cytology. The reproductive causes found in this study, subject to involuntary culling, were: clinical endometritis 1 Médicos Veterinários autônomos, Uberlândia, MG.