Euglena sp. is a freshwater microalgae producing beneficial metabolites in its biomass but the cultivation is not optimal yet for mass scale. The cultivation can be optimized with oxidative stress treatment like salinity. Salinity as an oxidative agent has a great role in maintaining optimum cellular metabolic rate so Euglena sp. growth can be optimal increasing lipid production for biodiesel and in carotenoid and chlorophyll as cell defense pigments that are beneficial in health. In this research, Cramer-Myers medium is used under 10 g/L (gram per liter) (0.281 M), 20 g/L (0.563 M), 30 g/L (0.845 M), and 0 g/L salinity level treatments in 7 days as the growth medium. Based on the research result, it shows that cell density decreases while biomass content, lipid content, chlorophyll content, and carotenoid content increase with salinity treatment in Euglena sp. culture with the optimal result met in 10 g/L salinity level treatment. Therefore, Euglena sp. culture with 10 g/L salinity level treatment can increase growth and metabolite production of Euglena sp. for biodiesel and pharmacy needs.
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