In vitro micropropagation of banana (Musa spp.) cv.virupakshi (Hillbanana) was studied. Suckers were collected from the germ plasm block of Jain R&D (originally established from the suckers from Palani Hills, Tamil Nadu) during summer. The sucker surface sterilized with 1% NaOCl for 30 min gave 100% survival without any contamination. Apical meristems that were isolated and cultured on MS based media supplemented with benzylaminopurine (BAP) 10.0 mg/l and IAA1.0 mg/l gave higher number of shoots (134.3 shoots/explant) within168 days (24 weeks). Kinetin 2.0 mg/l and NAA0.5 mg/l gave early rooting in just five days with 6.6 roots per plant. Observations were recorded after every four weeks up to six sub-culturing. Acclimatization was done in poly house, followed by shade house under 50% light conditions. The hardened plants when shifted to the field showed luxurious growth. The regenerated micro propagated banana plants were tested for genetic uniformity through 13 inter simple sequence repeat (ISSR) markers recommended by NCS-TCP, DBT. Profiles obtained by all the three ISSR primers namely, 834, 840 and 850, respectively exhibited similar banding patterns, which revealed the existence of genetic uniformity in micro-propagated plants.
The banana is one of the world's most important livelihood crops. Banana plants are principally infected by four virus species, (genus), (genus), (genus) and (genus). The objective of this study is to understand the codon usage pattern and phylogeny of coat protein gene in different banana bract mosaic virus (BBrMV) isolates. The BBrMV Coat Protein (CP) gene was amplified from BBrMV infected banana plant samples collected from different districts of Tamil Nadu and Karnataka, India. Six new BBrMV isolates were submitted to National Center for Biotechnology Information. Phylogenetic analysis and codon usage indices were studied along with other isolates of BBrMV. Phylogenetic analysis of CP genes shows that most of BBrMV isolates are closely related to each other except KF385484.1 and KF385478.1. Relative codon usage patterns among different BBrMV isolates were calculated by software CodonW 1.4.2. In BBrMV, codons with A-ended or U ended are the most preferential except the Leu and Gln whose optimized codons are CAG and UUG ending by G. The codon usage patterns of BBrMV isolates are principally influenced by mutational bias; however, compositional constraints along with mutational bias also play a major role.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.