A commercial micropropagation protocol for virupakshi (AAB) banana via apical meristem

Abstract

In vitro micropropagation of banana (Musa spp.) cv.virupakshi (Hillbanana) was studied. Suckers were collected from the germ plasm block of Jain R&D (originally established from the suckers from Palani Hills, Tamil Nadu) during summer. The sucker surface sterilized with 1% NaOCl for 30 min gave 100% survival without any contamination. Apical meristems that were isolated and cultured on MS based media supplemented with benzylaminopurine (BAP) 10.0 mg/l and IAA1.0 mg/l gave higher number of shoots (134.3 shoots/explant) within168 days (24 weeks). Kinetin 2.0 mg/l and NAA0.5 mg/l gave early rooting in just five days with 6.6 roots per plant. Observations were recorded after every four weeks up to six sub-culturing. Acclimatization was done in poly house, followed by shade house under 50% light conditions. The hardened plants when shifted to the field showed luxurious growth. The regenerated micro propagated banana plants were tested for genetic uniformity through 13 inter simple sequence repeat (ISSR) markers recommended by NCS-TCP, DBT. Profiles obtained by all the three ISSR primers namely, 834, 840 and 850, respectively exhibited similar banding patterns, which revealed the existence of genetic uniformity in micro-propagated plants.

In Vitro Clonal Propagation of Musa Sp. Cultivar Gopi: A Palatable Banana of Tripura, India

“…The optimum and effective treatment of cytokinin for fruitful shoot multiplication of the cultivar Gopi was recorded in MS medium supplemented with BAP 8mg l -1 during 42 days old culture (Table 1). However, previous reports on banana micropropagation demonstrated the use of MS medium fortified with BAP and IAA proving as the effectual combination and providing with the desired outcomes concerning rapid shoot initiation [15][16][17]. Several reports on tissue culture of banana have also been reported by different workers using cytokinin as a principal growth regulator for induction and multiplication of shoot bud formation [5,[18][19][20][21][22].…”

In vitro Propagation of Medicinally Valuable Traditional Banana Cultivar, Musa acuminata cv. Matti by Shoot Tip Culture

“…Kishor et al, (2017) used cocopeat and vermiculite in 1:1 ratio for optimum growth and development. Karule et al, (2016) reported acclimatization of plantlets in polyhouse, followed by shadenet house under 50% sunlight and the plants showed luxurious growth in field (Table 5) (Fig. 1-7).…”

Paclobutrazol Mediated Enhanced Multiplication of Musa Paradisiaca Linn. Cv. Poovan (Aab).

“…Poovan has been well expressed as all the explants proliferated in most of the media tested. Shoot tip explant has been identified as the preferred material for micropropagation for a wide range of banana cultivars such as 'Mehersagar' (Rahman et al,2006), 'Basrai' (Muhammad et al, 2007), 'Virupakshi' (Karule et al, 2016). However, immature inflorescence tip has also been employed for micropropagation in different cultivars (Resmi and Nair, 2007;Mahadevet al, 2011).…”