High-performance liquid chromatography (HPLC) has become an indispensable tool for modern analytical chemistry, and reversed-phase (RP) HPLC is one of the most widely used techniques for the separation and analysis of complex mixtures. In recent years, there have been numerous advances in RP HPLC method development, including the development of new stationary phases, improved column technology, and novel optimization strategies. These advances have led to increased speed, resolution, and sensitivity, as well as improved selectivity and reduced non-specific adsorption. Moreover, RP HPLC has found applications in a wide range of industries, including pharmaceuticals, food and beverage, environmental analysis, forensic science, and biotechnology. Despite its many benefits, RP HPLC method development is not without its challenges, including issues with column stability, sample preparation, and optimization. In this review article, we provide a comprehensive overview of the principles, optimization strategies, and recent advances in RP HPLC method development. We discuss the applications of RP HPLC in various industries and identify the common challenges and emerging trends in the field. Additionally, we evaluate the impact of RP HPLC on the accuracy, sensitivity, and selectivity of analysis and its potential to improve the quality and safety of products and the environment. Overall, this review highlights the importance of RP HPLC in modern analytical chemistry and provides insights and recommendations for researchers and practitioners who are interested in this technique. By exploring the latest advances, applications, and challenges in RP HPLC method development, we hope to inspire continued innovation and progress in this important field.
This paper proposes a high-performance liquid chromatography (HPLC) method for the simultaneous estimation of nortriptyline and gabapentin. The analytical conditions of the method were selected based on the chemical nature of the two compounds, and the method was validated through various studies including system suitability, specificity, linearity, LOD/LOQ, and recovery studies. The proposed method uses a Zorbax C18 column, a phosphate buffer with an acidic pH, and acetonitrile as the organic constituent of the mobile phase. The system suitability test evaluated the suitability of the chromatographic system for the analysis of the drug combination, and the specificity of the method was evaluated by assessing interference from excipients in the pharmaceutical dosage form prepared as a placebo solution. The linearity of the method was evaluated through linear regression analysis of the standard curve, and the LOD and LOQ values were determined by serial dilutions of nortriptyline and gabapentin stock solutions. The recovery studies were conducted to evaluate the degree of accuracy of the proposed method through known amounts of standard nortriptyline and gabapentin added to preanalyzed samples. Overall, the proposed HPLC method offers a reliable and accurate means for the simultaneous estimation of nortriptyline and gabapentin.
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