The prevalence of p53 positive samples of pterygium was 44%. The influence of sex and age on p53 protein expression in pterygium was not found. The increased proliferative acivity was present in the epithelium of pterygium. The expression of Ki-67 protein is associated with the expression of p53 protein in pterygium. The findings of our study support the thesis of pterygium as tissue growth disorder.
Introduction: In pregnancy-induced hypertension (PIH) there is a disorder in placental blood flow which causes intrauterine fetal hypoxia, and oxidative stress has a significant role in this condition. The aims of this research were to analyze the relation of thiobarbituric acid reactive substance (TBARS), as a marker of oxidative stress and absent end-diastolic flow (AEDF), as well as the relation of TBARS and cerebroplacental ratio (CPR). Methods: The research included 200 pregnant women in the gestation period from 28th to 40th, 100 were in a control group and 100 were with PIH. The CPR and TBARS were analyzed in all examined pregnant women. The CPR was calculated by dividing the Doppler indices of the middle cerebral artery by the umbilical artery. Results: Mean value of TBARS in the group with the PIH who had AEDF was in the interval of high values - 43.22 μmol/l. The result shows that through the application of Spearman’s coefficient, the correlation results in a statistically significant correlation between CPR and TBARS values: ρ = - 0.249, p = 0.0001. Conclusion: PIH has a very high level of oxidative stress, especially in pregnant women with absent end-diastolic flow in the umbilical artery and pathologic CPR. The Spearman’s test results in statistical significance and negative correlation, which means that in higher TBARS values, values of CPR are lower and vice versa, which indicates a possibility of clinical application of TBARS.
Introduction/Objective. The frequency of a sedentary lifestyle during pregnancy increases. This contributes to gestational weight gain and has a negative impact on health. This study researched the impact of prenatal exercise on gestational weight gain, blood pressure, and microcirculation in pregnant women who exercised and those who did not exercise. Methods. The study included seventy pregnant women with a normal pregnancy, who attended a psychophysical preparation program for childbirth for eight weeks. The control group (n=35) attended theoretical classes on childbirth, and the experimental group (n=35) attended prenatal exercises as well. Gestational weight gain, blood pressure, and nailfold capillary density were determined and compared between the two groups. Results. Gestational weight gain of 19.94 kg in non-exercising pregnant women was significantly greater than the gestational weight gain of 11.65 kg in pregnant women who exercised. Pregnant women who did not exercise had an increase in systolic (by 15.56 mmHg) and diastolic pressure (by 16.08 mmHg), which is significantly higher compared to pregnant women who exercised. In this group, systolic pressure increased by 2.5 mmHg, while diastolic did not change. A significant difference in the nailfold capillary density at the end of the prenatal program has not been determined. Conclusion. Prenatal exercise of moderate-intensity has a positive effect on gestational weight gain and the level of blood pressure in pregnant women. The nailfold capillary density has not differed significantly after the prenatal program in pregnant women who have exercised and in those who have not.
Introduction. The amniotic membrane is used in transplant surgery, ophthalmology and dermatology. Various methods have been developed to preserve amniotic membrane: hypothermic storage, cryopreservation, lyophilization. Transplantation of fresh amniotic membrane showed low inflammatory response. The efficient antibiotic solutions are carefully chosen for the hypothermic storage of amniotic membranes. The aim of this study was to compare the efficacy of two antibiotic solutions for the hypothermic amniotic membrane preservation and the structure of the amniotic membrane after the preservation process. Methods. Fifteen amniotic membranes were prepared and hypothermically stored in penicillin solution in BSS (2000 IU/ml) while the remaining fifteen in an antibiotic solution containing: benzylpenicillin (50 µg/ml), gentamicin (100 µg/ml), ciprofloxacin (200 µg/ml) and fluconazole (100 µg/ml). All amniotic membranes were microbiologically tested after preparation and after hypothermic storage for two weeks. Histological analysis of thirty amniotic membranes was performed after the process of preservation. Results. Fifteen amniotic membranes were sterile after hypothermic preservation in the penicillin solution. Also, fifteen amniotic membranes were sterile after hypothermic preservation in the solution of antibacterial agents (penicillin, gentamicin, ciprofloxacin) and antifungal agent fluconazole. The amniotic membrane had a normal structure and thickness of 35.33±11.03 mm. Conclusion. Both antibiotic solutions, one that contains only penicillin and one that contains multiple antibacterial agents and fluconazole, provide sterility of fresh hypothermically stored amniotic membranes for two weeks. In the preparation of fresh hypothermically stored amniotic membrane, a solution with multiple antibiotics is preferred. The normal tissue structure of the amniotic membrane was histologically confirmed after the process of preservation.
<p><span id="page13R_mcid298" class="markedContent"><span dir="ltr" style="left: 118.11px; top: 318.413px; font-size: 16.6667px; font-family: serif; transform: scaleX(1.09801);" role="presentation">The study concerns a 65-year-old female patient with pterygium of right eye and histologically verified </span></span><span id="page13R_mcid299" class="markedContent"><span dir="ltr" style="left: 118.11px; top: 338.413px; font-size: 16.6667px; font-family: serif; transform: scaleX(1.13244);" role="presentation">conjunctival intraepithelial neoplasia. The lesion was diagnosed as pterygium and treated surgically. </span></span><span id="page13R_mcid300" class="markedContent"><span dir="ltr" style="left: 118.11px; top: 358.413px; font-size: 16.6667px; font-family: serif; transform: scaleX(1.11182);" role="presentation">Histological examination of the excised material found the presence of conjunctival intraepithelial ne</span></span><span id="page13R_mcid302" class="markedContent"><span dir="ltr" style="left: 118.11px; top: 378.413px; font-size: 16.6667px; font-family: serif; transform: scaleX(1.09819);" role="presentation">oplasia. The significance of this case report is in pointing out the presence of morphological changes in </span></span><span id="page13R_mcid303" class="markedContent"><span dir="ltr" style="left: 118.11px; top: 398.413px; font-size: 16.6667px; font-family: serif; transform: scaleX(1.11778);" role="presentation">pterygium that may precede the development of squamocellular carcinoma of the conjunctiva and the</span></span><span id="page13R_mcid304" class="markedContent"><br role="presentation" /><span dir="ltr" style="left: 118.11px; top: 418.413px; font-size: 16.6667px; font-family: serif; transform: scaleX(1.0891);" role="presentation">necessity of histological examination of all excised pterygia.</span></span></p>
Recent studies indicate that placental and umbilical cord morphometry are the factors that may be associated with pregnancy complications, such as fetal growth restriction. Recently, placental and umbilical cord morphometry have been performed using digital image analysis. The aim of this study was to determine the morphometric parameters of placentas using digital image analysis. Material and methods: The digital imaging analysis of twenty placentas and umbilical cord were performed using Image Analysis LAS V4.3 software. Results: The length of the placentas was 191,77 mm ± 35,86 mm (mean ± standard deviation). The width of the placentas was 166,01 mm ± 19,01 mm. The placental surface area was 24495,13 mm2 ± 7038,86 mm2. The insertion of the umbilical cord to the placenta was central in 50 %, peripheral in 37,50 % and marginal in 12,5 % of analyzed placentas. The average distance of the umbilical cord insertion from the nearest placental margin was 38,89 mm ± 28,39 mm. The umbilical cord diameter at the insertion site was 21,16 mm± 5.69 mm. The diameter of the umbilical cord two centimeters from the insertion site was 12,36 mm ± 3,45 mm. Conclusion: Digital image analysis enables obtaining the objective morphometric parameters of the placenta and umbilical cord. The obtained morphometric parameters of the placenta and umbilical cord for our population are comparable to results of previous studies and open further placental research directions for the development of the screening method.
In pregnancy physical activity is considered as a preventive measure for diseases of the cardiovascular system. In studies, the data on microvascular adaptation in pregnancy are scarce. The prenatal program included 35 pregnant women who exercised and 35 women who did not. The shape of the capillaries of the nail skin fold was analyzed with capillaroscopy. At the beginning of the study, the shape of the capillary was normal in all pregnant women. After 8 weeks in 40.91% of pregnant women who exercised and in 47.06% of pregnant women who did not exercise, the shape of the capillary loop was abnormal with bending of the limbs, tortuosity of the limbs, and crossing of the limbs. After the 8th week of the prenatal program, no significant difference in the shape of the capillary loop of the nail skin fold was found between pregnant women who performed prenatal exercises and pregnant women who did not.
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