Figure 2. (A) Gigadalton-scale DNA brick structures made from 10,000 individual DNA oligonucleotides. The black arrow points to a conventional DNA origami structure as size comparison. Adapted with permission from ref 17. Copyright 2017 Springer Nature. (B) Hybrid λ/M13 phage-based DNA origami structure. The black arrow points to a conventional DNA origami structure lying on top as size comparison. Adapted with permission from ref 18.
Assembling hybrid substrates with nanometer-scale precision and molecular addressability enables advances in such distant fields as material research and biosensing. As such, the combination of lithographic methods with 2D DNA origami self-assembly [1-4] has led, among others, to the development of photonic crystal cavity arrays [2] and the exploration of sensing nanoarrays where molecular devices are patterned on the sub-micron scale [5-7]. Here we extend this concept to the third dimension through mounting 3D DNA origami onto nano-patterned substrates followed by silicification [8, 9] to provide mechanical and chemical stability. Our versatile and scalable method relying on self-assembly at ambient temperatures offers the potential to 3D-position any inorganic and organic components that are compatible with DNA architectures [10-13]. This way, complex and 3D-patterend surfaces designed on the molecular level while reaching macroscopic dimensions could supersede energy-intensive manufacturing steps in substrate processing.
Assembling hybrid substrates with nanometer-scale precision and molecular addressability enables advances in such distant fields as material research and biosensing. As such, the combination of lithographic methods with 2D DNA origami self-assembly has led, among others, to the development of photonic crystal cavity arrays and the exploration of sensing nanoarrays where molecular devices are patterned on the sub-micron scale. Here we extend this concept to the third dimension through mounting 3D DNA origami onto nano-patterned substrates followed by silicification to provide mechanical and chemical stability. Our versatile and scalable method relying on self-assembly at ambient temperatures offers the potential to 3D-position any inorganic and organic components that are compatible with DNA architectures. This way, complex and 3D-patterend surfaces designed on the molecular level while reaching macroscopic dimensions could supersede energy-intensive manufacturing steps in substrate processing.
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