DNA Origami Meets Bottom-Up Nanopatterning

Abstract

Figure 2. (A) Gigadalton-scale DNA brick structures made from 10,000 individual DNA oligonucleotides. The black arrow points to a conventional DNA origami structure as size comparison. Adapted with permission from ref 17. Copyright 2017 Springer Nature. (B) Hybrid λ/M13 phage-based DNA origami structure. The black arrow points to a conventional DNA origami structure lying on top as size comparison. Adapted with permission from ref 18.

DNA origami book biosensor for multiplex detection of cancer-associated nucleic acids

“…Micro and nanopatterning of the surfaces by the controlled placement of DNA origami sensors in a single-molecule nanoarray platform can also increase the sensitivity of the system and maximize the yield of the generated signal by reducing the false positive counts arising from random immobilization of single structures. 33–35…”

“…Micro and nanopatterning of the surfaces by the controlled placement of DNA origami sensors in a single-molecule nanoarray platform can also increase the sensitivity of the system and maximize the yield of the generated signal by reducing the false positive counts arising from random immobilization of single structures. [33][34][35] Moreover, by using artificial catalysts (macrocrowders such as polyethylene glycol (PEG) or diethylene glycol (DEG)) the sensitivity of the biosensor could be further improved and the detection limit of our structure could be lowered. 36,37 Modification of the locks with LNA could also potentially improve further binding of low concentration targets since LNA modification is known as a modification that increases stability and reduces the off-target effect.…”

Rational Fabrication of Nano-to-Microsphere Polycrystalline Opals Using Slope Self-Assembly

“…Additionally, the millimeter-scale, monolayer structures with high coverage obtained with this method can enable improvements in applications that use opals. By being able to fabricate structures with close to full coverage and no significant sphere stacking, with a method that is faster and more scalable than conventional approaches, it is possible to perform molecule printing experiments with high throughput and quality while circumventing the reliance on expensive top-down fabrication for biophysical and other single-molecule assays. , By being able to achieve 10 μm monolayers, which would not be possible due to sedimentation in methods like vertical deposition, we can enable inverse opals with permeability higher than what has been demonstrated due to larger pore sizes with smaller pressure drops. , These inverse opals have the potential to be used in passive evaporators, condensers, and high power density convection batteries …”

Optical characterization of DNA origami-shaped silver nanoparticles created through biotemplated lithography

“…Yet, there exist other possibilities to harness the spatial accuracy of DNA nanostructures in nanofabrication 39–42 to form optically resonant materials. 43–45 These include discrete DNA origami molds for casting metal and plasmonic nanoparticles with predefined dimensions in solution phase 46–48 and a combination of bottom-up-based DNA origami and common top-down lithography methods for surface-assisted nanopatterning.…”

“…conductive polymers, 22,23 carbon nanotubes, 24,25 fluorescent dyes, 26,27 plasmonic nanoparticles, [28][29][30][31][32][33][34] and superlatticeforming particles. [35][36][37][38] Yet, there exist other possibilities to harness the spatial accuracy of DNA nanostructures in nanofabrication [39][40][41][42] to form optically resonant materials. [43][44][45] These include discrete DNA origami molds for casting metal and plasmonic nanoparticles with predefined dimensions in solution phase [46][47][48] and a combination of bottom-up-based DNA origami and common top-down lithography methods for surface-assisted nanopatterning.…”