Presynaptic modulation has been associated mainly with calcium channels but recent data suggests that inward rectifier potassium channels (K(IR)) also play a role. In this work we set to characterize the role of presynaptic K(IR) channels in corticostriatal synaptic transmission. We elicited synaptic potentials in striatum by stimulating cortical areas and then determined the synaptic responses of corticostriatal synapsis by using paired pulse ratio (PPR) in the presence and absence of several potassium channel blockers. Unspecific potassium channels blockers Ba(2+) and Cs(+) reduced the PPR, suggesting that these channels are presynaptically located. Further pharmacological characterization showed that application of tertiapin-Q, a specific K(IR)3 channel family blocker, also induced a reduction of PPR, suggesting that K(IR)3 channels are present at corticostriatal terminals. In contrast, exposure to Lq2, a specific K(IR)1.1 inward rectifier potassium channel, did not induce any change in PPR suggesting the absence of these channels in the presynaptic corticostriatal terminals. Our results indicate that K(IR)3 channels are functionally expressed at the corticostriatal synapses, since blockage of these channels result in PPR decrease. Our results also help to explain how synaptic activity may become sensitive to extracellular signals mediated by G-protein coupled receptors. A vast repertoire of receptors may influence neurotransmitter release in an indirect manner through regulation of K(IR)3 channels.
Abstract-Recently, many educational institutions have acknowledged the importance of making laboratories available on-line, allowing their students to run experiments from a remote computer. While usage of virtual laboratories scales well, remote experiments, based on scarce and expensive rigs, i.e. physical resources, do not and typically can only be used by one person or cooperating group at a time. It is therefore necessary to administer the access to rigs, where we distinguish between three different roles: content providers, teachers and students.This paper reports on a conceptual model and technical design of a rig booking system that provides mechanisms for content providers and teachers to control and grant access to on-line remote laboratories. The design of the booking system is based on a requirements analysis carried out by the EC funded LiLa project in cooperation with international partners from the Global Online Lab Consortium, GoLC.
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