Background:
Epithelial ovarian cancer (EOC) is often challenging to diagnose, even though histological
examination. microRNA (miRNA or miRNA) is bound to the target messenger RNA (mRNA) and causing the mRNA
molecules are silenced. The identification of miRNA expression-based EOC subtypes is considered a critical means of
prognostication. So far, the studies on EOC subtypes have not been well characterized.
Objective:
This study aimed to confirm the existence of miRNAs in EOC and to assess their potential as clinical
biomarkers for EOC.
Methods:
We sampled 25 ovarian tumor tissues from patients with human ovarian tumors (17 malignant; 12 serous EOC,
five non-serous EOC, and eight benign ovarian tumors). miRNA microarray detection was performed to identify EOC
miRNAs. Real-time PCR was adapted for the validation of differentially expressed miRNAs detected by microarray
analysis was related to hybridization intensity.
Results:
The results confirmed that miRNAs exist in EOC, relative expression of EOC miRNAs demonstrated that
upregulation of miR-483-5p, and downregulation of miR-127-3p, and miR-532-5p were significantly expressed in the
malignant group than in the benign group at p ' 0.05. Besides, miR-483-5p could also distinguish EOC subtypes between
serous EOC and non-serous EOC, with a p ' 0.05.
Conclusion:
A comprehensive miRNA expression profiling can help to refine subtype classification in EOC, opening new
opportunities for identifying clinically applicable markers for improved stratification and diagnostics of ovarian tumors.
Prader–Willi syndrome (PWS) is a genetic disorder caused by the expression disruption of genes on the paternally inherited allele of chromosome 15q11.2-q13. Apart from clinical diagnostic criteria, PWS is confirmed by genetic testing. Methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) is one of the molecular techniques used to analyze this syndrome. This study aimed to evaluate the concordance of the test results of MS-MLPA with conventional techniques in the diagnosis of PWS in Thai patients. Forty leftover specimens from routine genetic testing (MS-PCR and FISH) were tested to obtain MS-MLPA results. By comparison, perfect concordance was shown between the result of MS-MLPA and those of conventional techniques. In conclusion, MS-MLPA is an accurate and cost-effective assay that can be used to confirm PWS diagnosis with explicit deletion of affected genes.
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