MicroRNA Expression Profiling of Epithelial Ovarian Cancer Identifies New Markers of Tumor Subtype

Rattanapan, Yanisa; Korkiatsakul, Veerawat; Kongruang, Adcharee; Siriboonpiputtana, Teerapong; Rerkamnuaychoke, Budsaba; Chareonsirisuthigul, Takol

doi:10.2174/2211536609666200722125737

Cited by 9 publications

(7 citation statements)

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“…24 MiR-127-3p was found to be a significantly underexpressed miRNA in epithelial OC, so it could be used as a biomarker for epithelial OC. 25 Bi et al suggested that overexpressed miR-127-3p targeted BAG5 to restrain epithelial OC proliferation and invasion. 26 Also, Xia et al indicated that miR-127 played an anti-cancer role in OC.…”

Section: Discussionmentioning

confidence: 99%

Hsa_circ_0015326 Promotes the Proliferation, Invasion and Migration of Ovarian Cancer Through miR-127-3p/MYB

Zhang¹,

Liu²,

Li³

et al. 2021

CMAR

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Background More and more evidences show that circular RNA (circRNA) has an important role in ovarian cancer (OC). Hsa_circ_0015326 is a newly discovered upregulated circRNA in OC, but its role and mechanism in OC have not been studied yet. Methods Quantitative real-time PCR was used to detect the expression of hsa_circ_0015326, microRNA (miR)-127-3p and MYB. The viability, colony number, cell cycle process, invasion, migration and apoptosis of cells were determined using cell counting kit 8 assay, colony formation assay, flow cytometry, transwell assay and wound healing assay. Moreover, the protein expression levels of metastasis, proliferation, apoptosis markers and MYB were assessed using Western blot analysis. The interaction between miR-127-3p and hsa_circ_0015326 or MYB was confirmed by dual-luciferase reporter assay and RNA immunoprecipitation assay. Xenograft tumors were built to explore the role of hsa_circ_0015326 in OC tumor growth in vivo. Results Elevated expression of hsa_circ_0015326 was identified in OC tissues and cells. Loss-of-function experiments suggested that silenced hsa_circ_0015326 inhibited the proliferation, invasion, migration, and promoted the apoptosis of OC cells in vitro, as well as inhibited OC tumorigenesis in vivo. Mechanically, hsa_circ_0015326 sponged miR-127-3p and miR-127-3p targeted MYB. The rescue experiments revealed that miR-127-3p inhibitor reversed the inhibitory effect of hsa_circ_0015326 silencing on OC progression, and MYB overexpression reversed the suppressive effect of miR-127-3p on OC progression. In addition, our data indicated that MYB expression was positively regulated by hsa_circ_0015326. Conclusion This study showed that hsa_circ_0015326 could facilitate OC progression by regulating the miR-127-3p/MYB axis, which suggested that it might become a potential target for the treatment of OC.

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Section: Discussionmentioning

confidence: 99%

Hsa_circ_0015326 Promotes the Proliferation, Invasion and Migration of Ovarian Cancer Through miR-127-3p/MYB

Zhang¹,

Liu²,

Li³

et al. 2021

CMAR

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“…Regarding the regulation of miRNA genes, a group of six chromosomes, 19 miRNAs clustered on chromosome 19, and seven clusters were up-regulated on chromosome 14, DNMT-inhibitor decitabine inhibitor, showing that miRNAs can be controlled by DNA methylation [26]. What's more, an overall, collective tumor-MiRNAs' suppressive effect has been suggested by Drosha and Dicer down-regulation, involving two enzymes in the processing of miRNA, being significantly connected with an early stage of ovarian cancer and poor prognosis [27,28].…”

Section: Microrna Dysregulation In Ovarian Cancermentioning

confidence: 99%

Epigenetic Events in Ovarian Cancer

Rattanapan¹,

Chareonsirisuthigul²

2021

Ovarian Cancer - Updates in Tumour Biology and Therapeutics [Working Title]

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Epigenetic aberrations are now well established in the development and progression of ovarian cancer, including DNA methylation, histone modifications, and microRNA dysregulation, and their progressive accumulation is correlated with the progression of the stage grade of disease. Epigenetic aberrations are relatively stable, linked to various subtypes of the disease, and present in circulating serum, representing promising diagnostic, prognostic, and pharmacodynamic biomarkers. Unlike DNA mutations and deletions, aberrant gene-repressive epigenetic changes, including DNA methylation inhibitors or histone-modifying enzymes, are theoretically reversible by epigenetic therapies. While no action against solid tumors, including ovarian cancer, has been shown in epigenetic monotherapies, preclinical studies indicate that they may be successful when used in conjunction with one another or with conventional chemotherapy, and combinatorial epigenetic therapy regiments are being investigated in cancer clinical trials. Improved interventions against this debilitating malignancy will provide a greater understanding of epigenetics’ role in ovarian cancer.

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“…The dysregulation of particular miRNAs can differ between subtypes of OC. For instance, the overexpression of miR-483 occurs in serous epithelial ovarian cancer (EOC), but does not occur in non-serous EOC [ 102 ]. As demonstrated in voluminous publications, miRNA dysregulation affects a variety of cellular processes that contribute to oncogenesis in a wide variety of cancers.…”

Section: Mirna Dysregulation In Cancermentioning

confidence: 99%

ROS and miRNA Dysregulation in Ovarian Cancer Development, Angiogenesis and Therapeutic Resistance

Stieg

Wang

Liu

et al. 2022

IJMS

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The diverse repertoires of cellular mechanisms that progress certain cancer types are being uncovered by recent research and leading to more effective treatment options. Ovarian cancer (OC) is among the most difficult cancers to treat. OC has limited treatment options, especially for patients diagnosed with late-stage OC. The dysregulation of miRNAs in OC plays a significant role in tumorigenesis through the alteration of a multitude of molecular processes. The development of OC can also be due to the utilization of endogenously derived reactive oxygen species (ROS) by activating signaling pathways such as PI3K/AKT and MAPK. Both miRNAs and ROS are involved in regulating OC angiogenesis through mediating multiple angiogenic factors such as hypoxia-induced factor (HIF-1) and vascular endothelial growth factor (VEGF). The NAPDH oxidase subunit NOX4 plays an important role in inducing endogenous ROS production in OC. This review will discuss several important miRNAs, NOX4, and ROS, which contribute to therapeutic resistance in OC, highlighting the effective therapeutic potential of OC through these mechanisms.

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MicroRNA Expression Profiling of Epithelial Ovarian Cancer Identifies New Markers of Tumor Subtype

Cited by 9 publications

References 0 publications

Hsa_circ_0015326 Promotes the Proliferation, Invasion and Migration of Ovarian Cancer Through miR-127-3p/MYB

Hsa_circ_0015326 Promotes the Proliferation, Invasion and Migration of Ovarian Cancer Through miR-127-3p/MYB

Epigenetic Events in Ovarian Cancer

ROS and miRNA Dysregulation in Ovarian Cancer Development, Angiogenesis and Therapeutic Resistance

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