In the Russian Federation, for the period from 2014 to 2019, the largest number of patients with brucellosis was detected among cattle – 55,591 heads and small cattle - 8,814 heads, which is 80.7 % and 12.8 %, respectively, of the total number of animals that respond positively to brucello-sis. Brucellosis of cattle was detected in 28 subjects of the Russian Federation, brucellosis of small cattle-in 8, brucellosis of horses in 9, brucellosis of pigs and deer (marals) in three subjects, respec-tively. In 2019, 397 cases of the disease were registered in the country.
The most common type of falsification of animal products is the substitution of raw materials of more valuable types with less valuable ones, including poultry meat. This paper presents the results of identification of undeclared chicken DNA in meat products using real-time polymerase chain reaction to detect falsification of products sold in Moscow and the Moscow region. As a result of PCR research, chicken DNA (Gallus gallus) was found in six out of ten samples of meat products, but semi-quantitative analysis excluded one meat product, since the amount of the desired component was less than one percent. All ten samples were also subjected to organoleptic evaluation, physical and chemical studies, luminescent microscopy, and the determination of safety indicators (the number of chemical elements, pesticides, antibiotics, and radionuclides). The results of comprehensive research did not reveal any deviations. Thus, the method of polymerase chain reaction allows you to determine the type of raw materials in the composition of minced meat products, finely ground semi-finished products, including those subjected to heat treatment. To accurately confirm the presence of falsification of meat products detected by PCR-RV, not only qualitative analysis, but also quantitative analysis is necessary.
The article reviews the diseases of cyprinid fish in the farm quarantined for aeromonosis and inflammation of the swim bladder. The etiology and symptoms of the disease are considered, and the pathoanatomical changes in fish. Modern methods of control and treatment of each age category of reared objects are considered. Preventive therapeutic feeding of farmed fish, vaccination, summer drying of ponds are offered. A veterinary and sanitary assessment of the farmed fish is given. Sick fish, if it has not lost its marketable appearance and meets the nutritional qualities, are allowed to eat people without restrictions. Non-market fish is sent to feed farm animals, poultry, fur-bearing animals in a boiled form or processed into fish flour. It is recommended to pay more attention to ecological methods of combating fish diseases – the use of polyculture for the effective production of marketable products (marketable fish); to increase the non-specific resistance of fish with probiotics; to use immunological methods, such as vaccination.
Leptospirosis is a dangerous zoonotic disease caused by pathogenic bacteria Spirochaetales of the genus Leptospira. To detect antibodies against Leptospira hardjo in the blood serum from 50 heads of vaccinated cattle and 40 heads of unvaccinated cattle, a comparative study was conducted using the methods of МАТ, ELISA and IHA. During the study of unvaccinated cattle, the coincidence between serological methods was 95 %, the tests are identical in specificity and sensitivity. Among vaccinated cattle in the MAТ with Leptospira Hardjo, 70 % of positive results were obtained, 58 % in IHA and 42 % in ELISA, a suspicious result was obtained in 4 % in ELISA and 9.5% in IHA. Suspicious animals in ELISA and IHA reacted in MAТ no higher than titer 1/100. Any of the presented methods of serological diagnostics is suitable for the control of this pathogen. But for a correct diagnosis, the history of vaccination of animals should be taken into account.
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