Artocarpus hirsutus Lam. is a tropical evergreen recalcitrant tree
species. The seed development is marked by the histodifferentiation
phase which is highly complicated by the interaction of a variety of
proteins involved in embryo expansion and differentiation. For a better
understanding of the physiology and biochemistry of this process,
protein expression profiling was carried out by SDS-PAGE followed by
LC-MS/MS technique. 410 proteins could be identified from the spectral
data belonging to different classes viz. cell division, metabolism,
stress, transport, folding, hormone metabolism etc. Simultaneous
analysis of Plant growth regulators (PGRs) by LC-MS/MS technique
revealed that IAA, GA4, trans-Zeatin (tZ), ABA, Jasmonic acid (JA),
Epibrasinolide (24epiBl) and salicylic acid (SA) were found to be in
elevated levels in the young embryos. However, proteome analysis only
showed JA biosynthetic proteins. Results indicate that most of the PGRs
involved in histodifferentiation process of recalcitrant embryo
development is provided by the maternal tissue rather than de-novo
synthesis in young embryo, a possible answer to a much debated question.
Comparison of seeds to analyse protein expression during
histodifferentiation in Artocarpus hirsutus with Phaseolus vulgaris
showed almost similar processes are happening in both the species.
However the accumulation of some proteins showed different pattern.
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