Proteomic Analysis in the Histodifferentiation Process during the Recalcitrant Seed Development of Artocarpus hirsutus. Lam

Abstract

Artocarpus hirsutus Lam. is a tropical evergreen recalcitrant tree species. The seed development is marked by the histodifferentiation phase which is highly complicated by the interaction of a variety of proteins involved in embryo expansion and differentiation. For a better understanding of the physiology and biochemistry of this process, protein expression profiling was carried out by SDS-PAGE followed by LC-MS/MS technique. 410 proteins could be identified from the spectral data belonging to different classes viz. cell division, metabolism, stress, transport, folding, hormone metabolism etc. Simultaneous analysis of Plant growth regulators (PGRs) by LC-MS/MS technique revealed that IAA, GA4, trans-Zeatin (tZ), ABA, Jasmonic acid (JA), Epibrasinolide (24epiBl) and salicylic acid (SA) were found to be in elevated levels in the young embryos. However, proteome analysis only showed JA biosynthetic proteins. Results indicate that most of the PGRs involved in histodifferentiation process of recalcitrant embryo development is provided by the maternal tissue rather than de-novo synthesis in young embryo, a possible answer to a much debated question. Comparison of seeds to analyse protein expression during histodifferentiation in Artocarpus hirsutus with Phaseolus vulgaris showed almost similar processes are happening in both the species. However the accumulation of some proteins showed different pattern.