We conducted a study on the pathogenicity of some ferments (DNA-ase, lecithinase, phosphotase) and hemolytic activity of Klebsiella, Pseudomonas aeruginosa, Salmonella and E.coli, isolated from water of the main water reservoirs in the Rostov region (Tsimlyansk Reservoir, Lower Don). It was found that the growth of the antropotehnogenic impact on the aquatic ecosystems contributed to the increase in the number of bacteria containing pathogenicity enzymes, that is to the strengthening of the adaptive properties of the studied bacteria.
The used methodology of the scientific substantiation of indicators is in the establishment of the conformity of laws of vital activity of indicator and pathogenic microorganisms in the real conditions of the action of the complex of factors, including disinfecting agents. In the one water sample simultaneously there were determined both the general indicator (GICB), thermotolerant (TTCB), glucose positive (GPCB) coliform bacteria, E.coli. On the base of long-term research in the various regions of the Russian Federation, as well with bearing in mind the analysis of domestic and foreign data, comparing the water quality and the incidence of intestinal infections in population it is recommended to use the index of determination of the total number glucose positive coliform bacteria (GPCB), which brings together a much broader range of bacteria of the Enterobacteriaceae family in comparison with total coliform bacteria (TCB) and thermotolerant coliform bacteria (TTCB) and warrants the absence in the test volume of water as an indicator lactose positive (E.coli, TCB, TTCB) and pathogens (salmonella) and potentially pathogenic bacteria which do not ferment lactose. Proposed index of GPCB is shown to allow to assess epidemiological risks not only more accurate, but also more efficiently without increasing the cost performance of bacteriological research.
The increasing chemicalization of production and life leads to the pollution of water bodies by chemicals, the effect of which on the micro - and macro - organisms is poorly understood. This section of the study in sanitary bacteriology is becoming ever more topical and is an important task of modern hygienic science. One of complicacies of the study of the problem is related with the fact that the presence of only experimental data fails to be sufficient, as the impact of any given chemical substance on different bacteria in the experiment does not mean that under natural conditions, similar results will be obtained. One reason for this may be the inhibitory effect of the given chemical on biological properties of bacteria, while in field conditions in the water several chemicals interacting with each other can exist. In this regard, the aim of the work was to assess the indicator value of sanitary and microbiological indices of epidemic hazard of water use in conditions of chemical pollution of surface water bodies.
Introduction. The main task of treating sewage sludge is to obtain the final product, the properties of which would ensure the possibility of its utilization and minimize the damage to the environment. The sanitary and hygiene characteristics of the sediment are assessed by the presence of pathogenic microorganisms and helminths eggs. The degree of disinfection of the sediments is controlled according to SanPiN 2.1.7.573 - 96. The objective of the work. Determination of optimal doses of caustic lime for disinfection of microbial contamination of sewage sludge of household wastewater of sewerage cleaning facilities (SCF) and liquid manure of the swine complex. Material and methods. The object of the study were microbial communities of the sludge of the SCF household wastewater and liquid manure of the swine complex. Standard microbiological research methods were used in the work according to MU 2.1.5.800 - 99 and MR N FTs/4022. For representativeness, analyses were performed in triplicate. Results. Caustic lime was added in the amount of 1 to 10% of the volume (mass) of the sludge under study into the sludge of household wastewater (humidity of 97 - 98%). The contact time was 0.5 hours, 1 hour, 3 hours. At a concentration of caustic lime of 5 and 6%, the absence of microflora was noted after 1 hour, at a concentration of caustic lime 8 - 10% - after 0.5 hours. After 3 hours, for any amount of caustic lime (from 1 to 10%) in the native sludge of wastewater sewerage, all the microorganisms under study were not shown. Into the liquid manure (humidity less than 85%), caustic lime was added in the amount of 4 - 10% of the volume (mass) of the material being examined. The exposure was of 1 and 3 hours. The complete death of microbes at any exposure was observed at a concentration of caustic lime 9 and 10%. Conclusion. The investigations have shown the dynamics of death of microorganisms in studying the impact of caustic lime on both native and sterilized material to be the same. The total death of microorganisms during the treatment of sludge from household wastewater was observed at a concentration of caustic lime of 7 - 9%. The complete death of microbes in liquid manure (native and sterilized) at any exposure was observed at a concentration of caustic lime 9 and 10%.
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