Farmers should focus on milk quality over quantity because milk that contains unsuitable components and/or antibiotic residues, or has a high somatic cell count, cannot be used in food production and thereby results in reduced milk yield. One of the main problems affecting the ultimate milk yield of dairy cows is mastitis. This disease is the most serious economic and health problem associated with dairy cow herds and is a major reason for excessive culling. Therefore, many studies have addressed this problem to further our understanding of the agents causing mastitis and their classification and virulence factors. This review summarizes the current knowledge regarding mastitis prevalence, the characteristics of its main causative agents, and the effects of mastitis on dairy production. The review also intends to provide guidance for future studies by examining external effects influencing dairy production in cows under field conditions.

The aim of this study was to describe and analyze effects of parity, stage of lactation, milkability (3 groups of cows with differing peak flow rates), time of milking, and quarter position on milk production and milk flow measures at udder and quarter levels. Particular emphasis was put on changes to the decline phase and in duration of overmilking. More than 75,800 quarter milk flow curves and more than 19,300 udder milk flow curves obtained from 38 cows throughout lactation were analyzed. Stage of lactation significantly influenced all studied variables at both udder and quarter levels. At the quarter level, the duration of decline phase and the decline ratio (decline phase as a percentage of milking time) decreased from mo 1 to 2 and then gradually increased as lactation advanced. In contrast, at the udder level, duration of decline phase decreased throughout lactation but beginning at mo 2, the decline ratio increased as lactation advanced. The duration of the overmilking phase of quarters increased from mo 1 to 3 and then decreased in the course of lactation. Parity did not influence peak and average flow rates, the duration of increase phase, or the decline ratio at either udder or quarter levels. All milk flow measures were higher during morning milking except the duration of increase and decline phases at the quarter level and the duration of increase phase at the udder level. Milk yield and the duration of increase phase were not affected by milkability at either level. Quarters from udders with high milkability had longest duration of decline phase and the shortest overmilking phase. Milkability did not influence duration of the decline phase at the udder level. Quarter position influenced all measured variables of milk yield and milk flow. Rear quarters had significantly higher milk yield, longer time of milking, higher peak, and higher average flow rates than front quarters. Front quarters had shorter duration of increase and decline phases than rear quarters. The duration of the overmilking phase was almost double for front quarters. There were also differences in measured flow rates between left or right quarters on respective front or rear positions. Measured characteristics reported in this study may be important in setting default parameters in automated milking systems.

The aim of this study was to test if the opioid antagonist naloxone has a beneficial effect on normalization of oxytocin (OT) release during repeated milking of cows in unfamiliar surroundings. One control milking without naloxone treatment in all cows was performed in the familiar parlour. For four successive evening milkings, cows were transported to, and milked in, the operating theatre of the research station without (control group) or with naloxone administration (1 mg/kg BW) (naloxone group) before milking. After cessation of spontaneous milk flow, but not before 3 min of milking, vaginal stimulation was applied for 2 min. After milk flow ceased again, 10 IU of OT was injected intravenously to remove the remaining milk including residual milk. Milk flow was recorded continuously and blood samples were collected via a jugular vein cannula at 1-min intervals from 1 min before the start of milking until i.v. injection of OT. The inhibition of milk ejection and its normalization during repeated milking in unfamiliar surroundings was not influenced by naloxone treatment. Concentrations of cortisol and β-endorphin during control milking and all relocations were similar in the naloxone and control groups, although their concentrations were higher after relocations than in the control. Therefore, a role of endogenous opioids in the inhibition of milk ejection in unfamiliar surroundings could not be demonstrated. In addition, the effect of exogenous ACTH1–24 (8 IU, i.v.) on the release of cortisol related to the response of cows milked in unfamiliar surroundings was studied. Cows with totally inhibited milk ejection in response to vaginal stimulation during milking after first relocation had numerically, but not significantly lower cortisol levels (8·8±3·4 ng/ml; AUC/min) in response to ACTH than did cows with at least partial milk ejection (38·7±12·9 ng/ml). Thus animals with a higher adrenal response to ACTH seemed to have less severe inhibition of milk ejection.