This study aimed to investigate phenotypical and functional characteristics of dendritic cells (DCs) generated from monocytes of peripheral blood of healthy donors and cancer patients.Material and methods. DCs were used as natural adjuvants with antitumor vaccine as a part of combined treatment scheme for lung cancer patients. Phenotypical and functional characteristics of DCs were study using flow cytometry and real-time PCR.Results. We have found that in lung cancer patients generated DCs had moderate level of maturity and demonstrated more pronounced tolerogenic features in contrast to DCs of healthy donors (patients DCs had higher mRNA expression levels of suppressive molecules TGF-β and IDO, and secreted lower amount of bioactive IL-12 protein). Expression of CCR7 gene was particularly on the normal level in DCs of cancer patients which indicates on saving of migratory properties of these cells. Expression level of DC maturity marker CD83 increased after each subsequent vaccine administration, while the levels of TGF-β, IL-10 mRNAs to the end of vaccine therapy course decreased to the level observed in healthy donors DCs.Conclusion. Thus, the study of biological characteristics of DCs will help to improve and develop the most effective protocols for rational use of DC vaccines. These data indicate the need for further optimization of technologies of DC generation in patients with lung cancer with emphasis on the stimulation of Th1-polarizing properties by increasing cytokine-secreting potential.
Background. This work is а comparative study of survival in patients with stage III A non-small cell lung cancer (NSCLC) treated with neoadjuvant chemoradiotherapy (NCRT), neoadjuvant chemotherapy (NCT) and neoadjuvant chemotherapy and adjuvant radiation therapy (NCT + adjuvant RT). Material and methods. Three groups of 65 people were selected. The first group received NCT, the second group -NCT, and the third group -NCT + adjuvant radiation therapy. The NCRT group received radiation therapy of 30 Gy. Survival was assessed using the Kaplan and Mayer scale and according to the log rank criteria.Results. The statistical criterion for NCRT in relation to NCT was -3.51279, r=0.00044. The statistical criterion for NCRT in relation to NCT + adjuvant RT was -2.88568, r=0.00391. Less reliable dependence was observed between NCT and NCT + adjuvant RT (the statistical criterion =1.809508, p=0.070307). Conclusions. Performing NCT, NCRT or NCT + RT and the status of complete morphological response (CMR) during the revision of histological material, it is possible to predict survival for a period of up to 36 months. NCRT is the optimal method of treatment.
The aim: The aim of this study was to investigate disease-free and overall survival in patients with stage IIII A NSCLC after neoadjuvant chemotherapy, neoadjuvant chemoradiotherapy, and neoadjuvant chemotherapy.
Materials and methods: For the study, 3 groups of 65 people were taken. All patients underwent radical surgery in the form of lobectomy, bilobectomy or pneumonectomy. All groups received 3 or 4 courses of neoadjuvant chemotherapy according to the scheme Docitaxel (Paclitaxel) and Cisplatin (Carboplatin), while groups II and III also received, respectively, neoadjuvant and adjuvant radiation therapy in the amount of 30 Gy to the chest and mediastinum. Survival was assessed using the Kaplan and Mayer scale.
Results: 1. At a survival period of 6 months, the results of relapse-free survival in the NCRT and NCT groups do not differ. 2. From 12 months to 5 years, the highest recurrencefree survival rates are observed in the NHL group. 3. The overall five-year survival rate in the main group was 28.1±5.9%, in the NCT group - 10.4±3.8% and - 5.8±2.0% in the NCT + RT group.
Conclusions: The best recurrence-free survival results are observed in the NHLT group. Also, the largest number of cases of molars morphological response, correlated with the highest survival rates, also occurs in the NHL group.
Background: The purpose of this study was to experimentally assess the dosimetric impact of leaf interdigitation for NSCLC sliding window IMRT (dMLC) plans in Monaco TPS. Methods: 20 NSCLC patients (one to four isolate targets) previously treated were re-planned for dMLC with and without leaf interdigitation. Elekta synergy linear accelerator was used in this study. The treatment plans were evaluated on the following dosimetric variables: conformity index, homogeneity index and the normal lung sparing. Results: For one isolate target, the plan quality was equivalent with and without leaf interdigitation (p>0.05). For multiple targets, leaf interdigitation could improve the conformity index obviously (p<0.05). Meanwhile, leaf interdigitation showed well sparing for the normal lung (p<0.05). Conclusion: The study shows that leaf interdigitation can improve the plan quality for multiple isolate targets NSCLC dMLC plans in Monaco TPS.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.