Perosis is one of the most common leg pathologies in broiler chickens, during the period of intense weight gain – at the age of 14–35 days. Due to manganese deficiency, the number of sick birds can reach up to 5% of the flock. These studies were carried out in order to establish changes in some indicators of protein, macro- and micromineral metabolism in the blood serum of clinically healthy broiler chickens and birds with perosis at 14, 21 and 28 days of age. A batch of 2,000 Cobb-500 crossbred broiler chickens was selected. Two groups of chickens were directly involved in the research, in which blood was taken at the age of 14, 21 and 28 days: healthy birds and those with perosis signs. Clinical studies showed that 8.0% of chickens on 28th day suffered from perosis. Trace mineral biochemical parameters of serum and blood of broiler chickens with perosis on the 28th day of life significantly differed from those of healthy birds (manganese and zinc). It was found that on the 28th day of life the weight of chickens with perosis was reduced by 42.7%, causing a loss of weight 88 kg per batch of 2000 birds, with a consumption of feed 140 kg. The obtained data will allow the development of early perosis prevention schemes in broiler chickens, which will help manage production losses and increase its profitability. On farms, to prevent the occurrence of perosis, it is necessary to take into account the technological factors of the production of compound feed. Also, an increase in the level of total protein and albumin in serum in the blood may indicate inflammatory processes and dehydration of the body. Therefore, it is better to site a sick bird separately for rearing or hand over to a sanitary culling.
Trace elements in the body of the bird are in small quantities, but their role in productivity remains important. In order to get a quality carcass at the slaughterhouse, you need a constant supply of nutrients and, last but not least, microelements. Zinc and Manganese are essential trace elements that affect the growth and development of birds. And their deficiency in the body has a negative effect on the productivity of birds. In the diet of broilers trace elements are mainly added with a premix in the form of inorganic salts (sulfates, carbonates). However, these micronutrient compounds in the body of the bird are insufficiently absorbed, and increasing the dose of their introduction can cause toxicosis. Therefore, in order to prevent microelementosis, it is more appropriate to use organic compounds (chelates), which have a much higher level of bioavailability and less release into the environment. This review article describes the prophylactic efficacy of Zinc and Manganese chelates in micronutrient deficiencies in broiler chickens and laying hens. With a deficiency of a particular trace element develop metabolic disorders and other pathologies (perosis, etc.). Based on the presented data, it can be concluded that the use of organic forms of trace elements in poultry feeding is a better alternative to inorganic sources, as they can be used in smaller quantities. But due to their greater bioavailability, they are better absorbed, which has a positive effect on the productivity of broilers. Since knowledge about the use of chelated forms of trace elements in comparison with inorganic forms (salts, sulfates, etc.) in the diet of poultry is still poorly understood, it is necessary to further conduct research to prevent micronutrient deficiencies in these compounds.
The rapid growth of demand for poultry products requires its sufficient production by specialized farms of various forms of ownership. However, such production needs are not always adequate to the selection approach, the incubation component, the basic requirements of veterinary and sanitary and zoohygienic support, breed and age characteristics of keeping and raising poultry. Therefore, one of the crucial components of obtaining biologically complete, high-quality and fast-paying products of the poultry industry, including all stages of its production, is human support of the main links of ontogenesis (development after birth) of the bird. The issue of not only the creation of the genetic potential of the parent bird of different species and areas of productivity, but also the provision of veterinary and sanitary conditions for their maintenance, breeding and breeding remains relevant. However, the current economic conditions have forced the heads of enterprises and veterinary departments to some extent bypass the planned laboratory tests of feed, water and blood, which, although not complete, but informative enough to trace the main periods of growth and development of the bird. Slight deterioration of the mode and quality of feeding, changes in the parameters of the microclimate are reflected in changes in blood parameters. And what about the spoilage of feed, water, violation of veterinary and sanitary maintenance of poultry: the lack of preventive treatments with vitamin-mineral, hepatoprotective and enzyme preparations, pre- and probiotics. Which can lead to metabolic disorders in poultry. Which can occur due to disorders of protein, lipid, carbohydrate, vitamin, macro- and micromineral metabolism. As a result, there are significantly popular diseases such as: uric acid diathesis, cannibalism, osteoporosis and osteomalacia, perosis, rickets, obesity, E-hypovitaminosis. Further reducing productivity, which leads to large economic losses on farms.
Culling due to poor condition, weight loss, mortalityand, as a result, economic losses due to perosa, accordingto various estimates, 1-5% of the group of birds. Due to thedysfunction of the limbs, the bird can suffer from a numberof diseases. Lack of essential nutrients play an important rolein the development of perosis in poultry. Often in farms dueto cost savings, reduce the cost of feed for broilers, which inturn can lead to metabolic disorders in poultry. Therefore, thediagnosis of these disorders requires new approaches. Modernscience is increasingly inclined to non-invasive diagnosis ofmetabolic pathologies. In human medicine, nails and hair areused to study persistent micronutrient deficiencies. And inveterinary medicine in the study of micronutrient deficienciesas a method of non-invasive diagnosis using animal hair.According to the results of our research, theconcentrations of microelements (Zn, Mn) in the feathers of14, 21 and 28-days-old clinically healthy and feathery broilerchickens were determined. On day 14 of the disease, theconcentration of Zinc and Manganese in broiler chickens withperosis was 65.6 ± 2.73 and 17.4 ± 0.79 μg / g, which wasprobably higher than in clinically healthy birds 46.5 ± 1.57(p <0.001) and 10.9 ± 0.80 (p <0.001) μg / g. Analyzing the indicators of micronutrients in the clawsof patients with feathers of broiler chickens of 28 days ofage, the content of Manganese was 10.2 ± 0.51 μg / g, whichis probably less than in clinically healthy birds 15.7 ± 0.97μg / g (p < 0.01). The concentration of zinc in the claws ofhealthy birds is 127.6 ± 3.65 μg / g, which tended to increasecompared to that in healthy birds - 98.3 ± 5.40 μg / g.The results of the analysis show that in birds with signs ofperosis, Zinc and Manganese accumulate in higher concentrations in the feathers compared to clinically healthy chickens. Inturn, the concentration of these elements in the claws in chickens with feathers relative to healthy birds of the group decreases. Key words: trace elements, non-invasive diagnostics,metabolic pathologies, avian.
The results of the application of a complex of Zinc and Manganese chelates to protein, macro- and micro-mineral exchanges in the body of broiler chickens of the Cobb 500 cross-breed are presented. The administration of Zn and Mn chelates in a dose of 0,2 ml/l for chicken broilers of 23 days of age (1st experimental group) during 14 days influenced the increase in the total protein content to 35,3±0,8 g/l, which is 8 % higher compared with the control – 32,5±0,7 g/l (p<0,01 ) The concentration of total Calcium in serum of broiler chickens 37-day-old (end of trial) was higher in the first experimental group by 7,3 % (2,05±0,06 mmol/l) compared to control – 1,9±0,03 mmol/l. In the second group, where chelates were administered in a dose of 0,4 ml/l of water, the Calcium content was 2,0±0,02 mmol/l, which is 5 % more than the control group. The two-week application of Zn and Mn chelates also led to an increase in the level of inorganic Phosphorus in the serum of the first group to 2,6±0,12 mmol/l (+ 11,5 %) compared with the control group. After administration of chelates Zn and Mn at doses of 0,2 ml/l water, the Zinc content was higher by 4,3% (23,5±0,2 μmol/l) compared to control – 22,5±0,34 μmol/l (p<0,01). In the second group, where the dose of chelates was 0,4 ml/l, the concentration of Zinc was 23,6±0,16 μmol/l, which is more than 4,6 % for control (p<0,01). At the end of the study, the content of Manganese in serum of broiler chickens in the first group was 1,9±0,07 μmol/l (p<0,05), which is 10,4% higher compared with the control – 1,7±0,06 μmol/l (3rd sampling). The level of this element in the second group was 2,0±0,08 μmol/l (p<0,01), which is higher by 16,7 % relative to the control. At the end of the experiment, the coupon concentration in the first and second experimental groups was 12,6 and 9,3 % higher, compared with control, 7,0±0,37 μmol/l. In turn, the serum Ferrum content of the first group was greater by 9,3 % (19,3±0,69 μmol/l), and the second by 6,7 % (18,8±0,47 μmol/l) compared to the bird of the control group. Key words: chicken broilers, chelates, Zinc, Manganese, Cuprom, Ferrum, metabolism.
Perosis is a common metabolic disease of industrial birds, especially broiler chickens. It leads to a violation of the balance of biotic substances in the body of chickens, which is clinically manifested by the curvature of the limbs, reduced mobility, and, consequently, reduced profitability of meat production. Prevention of perosis is possible provided that chickens receive a sufficient amount of manganese in a biologically available form. Studies were conducted to determine the efficiency of use of manganese chelates (pantothenate and lysinate) for prevention of perosis in broiler chickens. Efficacy was confirmed by examining changes in the clinical state, indicators of protein and mineral metabolism, as well as meat productivity of birds. For the experiment, broiler chickens of the Cobb-500 cross were taken at the age of 14 days. The birds of the control group received a standard diet, and the chickens from two experimental groups additionally received manganese pantothenate and lysinate with water during the critical period for the development of perosis – 14–28 days old. After 14 days of administration of manganese pantothenate and lysinate, the weight of the experimental birds at the age of 28 days was greater by 133.6 g (+11.0%) and 142.2 g (+11.7%), respectively, in comparison with poultry of the control group. Additional provision of manganese pantothenate and lysinate to chickens of the experimental groups contributed to an increase in the blood serum total protein concentration by 11.0% and 12.8 %, albumin – by 10.1% and 8.2%, magnesium – by 8.1% and 9.0% and manganese – by 29.6% and 26.9%, respectively, compared with indicies of the control group birds. The use of manganese chelates in the form of pantothenate (0.2 mL/L of water) and a lysinate (0.5 mL/L) during the 14–28th days of broiler chickens’ rearing provides 100% prevention of perosis. This reduces the death of broiler chickens, increases body weight, and, as a result, significantly increases the profitability of meat production.
The article presents the results of the use of the vitamin-amino acid chelates (pantothenates) of Zinc and Manganese in order to study their effect on some indicators of avian metabolism. The studies were started on 14-day Cobb 500 broiler chickens in the poultry farm of the Bila Tserkva National Agrarian University Training and Production Center. For this purpose, 3 groups poultry were formed: two experimental ones (Zinc and Manganese chelates were fed to the main diet with water) and a control group (50 heads each). During the experiment (14, 21, 28 days), weighed chickens followed by blood sampling for biochemical study. In the group where the chelates were drunk at a dose of 0.2 ml/l of water over the next 14 days, there was a tendency to increase the absolute weight gain in broiler chickens to 943.0 ± 25.94 g (770–1073), compared with the control – 883.2 ± 24.64 g (740–1140). The bird, which was given chelates at a dose of 0,1 ml/l of water for 14 days, increased the content of total protein in the serum by 10.3% compared to the beginning of the study, and was 30.9 ± 0.71 g/l (P < 0.001). In the second group (chelate dose of 0.2 ml/l water), this indicator increased by 11.2% and amounted to 30.2 ± 0.82 g/l (P < 0.01). At the end of the experiment (28 days of cultivation), the albumin content in the blood of the birds of the first and second experimental groups was 18.6 ± 0.36 and 18.7 ± 0.37 g/l (P < 0.001 before the first selection). Manganese concentration in broiler chickens of the second experimental group on the 28th day was 3.6 ± 0.28 μmol/l and was higher than the control (2.7 ± 0.25 μmol/l; P < 0.01). At doses of 0,1 ml/l of water (1st experimental group), there was a tendency to increase the amount of this trace element in serum to 3.2 ± 0.16 μmol/l (1.4–4,0). After 14 days of chelating, Zinc content in the serum of chickens of the first experimental group increased by 20% and amounted to 27.9 ± 0.60 μmol/l, in its second – concentration increased by 25.8% (28.3 ± 0.76 μmol/l; P < 0.001) compared to the start of the experiment. Therefore, the use of the vitamin-amino acid chelates of Zinc and Manganese for 14 days at a dose of 0,1 and 0.2 ml/l of water contributed to the increase in weight gain, increase of total protein, Manganese and Zinc in the serum of broiler chickens.
Depending on age and live weight in the bird, blood can be taken in different places: by performing a puncture of the shoulder (subclavian), jugular, medial tibial veins, of the occipital sinus, of the heart, and through decapitation in the day-old young. But not all of these methods are practical and suitable for all types and periods of time in the poultry. The article describes two practical methods of life-time selection of blood in chickens-broilers of the cross-breed COBB-500 of different ages. Blood in day-old chicks is taken for the purpose of early diagnosis of deficiency of micro-and macro elements. In chickens that were hatched after 12 hours at a mass of at least 30 grams of blood, they were taken from a right jugular vein with an insulin syringe of 1 ml and then received 0,5-0,6 ml of whey individually from each chicken. Thanks to this, the chick remains alive and after 7 days it allows you to take blood again for further research. A subcutaneous vein subunit for taking blood in broiler chickens after 7 days and up to 17 days is better to use a syringe of 2 ml, as this is less injurious to the vein, but it is better to carry out this procedure with the assistant. It is more practical to make blood collection in chickens from 18 to 42 days using an injection needle with a pink cannula (18 G) and a polypropylene tube with a tufted lid that will allow it to remove up to 5 ml of blood from one bird and get enough serum for it biochemical studies. An important stage in the diagnosis of internal bird diseases, in particular metabolic etiology, is blood research. This allows us to diagnose the subclinical stages of illness associated with an imbalance of metabolic processes in an organism of productive and exotic birds. Therefore, one of the important measures in the establishment and confirmation of the diagnosis, as well as the study of the effectiveness of therapeutic treatments – is the selection of blood. Anatomical features of the body structure of various types of agricultural and exotic birds require the modification and modernization of blood selection technologies and make adjustments to the selection of the appropriate sites for manipulation. Blood in the bird collapses fast enough – 20-30 seconds, which makes it impossible to remove enough of it in young birds. In order to obtain the required volume of quality blood and its serum, it is necessary to take into account the age, physiological and productive qualities of birds. The most recent features were the development of new and improved existing methods of blood sampling in poultry. In the bird, blood can be taken by performing a puncture of the shoulder (subclavian), jugular, medial tibial veins, of the occipital sinus, of the puncture of the heart, and of the decapitation in a day-old youngster. The bird has a relatively small percentage of blood volume by weight, approximately 6-7,5%. The amount of blood that can be taken will depend on the weight of the bird, the skill of the researcher and the rate of blood coagulation. It is not necessary to take more than 1% of the blood from the body weight or 10% of the total blood volume, and the next selection is desirable to do not earlier than 14 days. Also, after the selection, hematoma may develop, which may lead to vascular collapse, so it is advisable to introduce warm isotonic solutions. Blood was taken with a 1 ml insulin syringe with a removable needle (29 g) from the right jaw vein. The jugular vein is the largest peripheral vein in the bird, in smaller species and chickens, this may be the only large enough place to select a significant amount of blood for diagnostic testing. The puncture was carried out in day-old chicks, 12 hours after hatching. Before the blood was taken, a clinical examination was performed, and weighing chickens. In those whose body weight was less than 30 grams blood was not taken. Fixed the chick in the left hand a little while turning to the left side, holding his neck between the index and the without limbs, pressing the chicken body with his thumb to the palm, thus best visualizing the jugular vein. At the site of the puncture, a fluff was pulled out and rubbed with 70% ethyl alcohol. Then gently at an angle of 10-20 ° the needle was injected into the vein and the blood was drawn slowly. As a rule, when a needle is correctly placed in the vein, the blood begins to fill the syringe reservoir. When selecting a syringe, use the thumb and forefinger, and slowly pull the syringe piston gently without tilting the needle. If the blood does not enter the syringe, the beveled edge may be against the vein or the needle may get stuck. Gently release the pressure on the piston and slightly bend the tip. Injecting needles, needleless needles or syringes of 2-10 ml may be used for venous puncture. To prevent blood coagulation, the lumen of the needle can be pre-moistened with a 5% solution of heparin. Blood can be taken from glass, polypropylene or fluoroplastic test tubes. Key words: broilers chickens, blood selection, jugular vein, subcrine vein.
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