Objective. To study the possibility of production of physiologically active substances by the as-sociation of micromycetes Trichoderma harzianum 128, which is used for enrichment composted substrates based on chicken litter. Methods. Microbiological, physiological, accumulative thin lay-er chromatography, high-performance liquid chromatography (HPLC / MS). Results. T. harzianum 128 produces a significant amount of physiologically active growth stimulanting substances. Soak-ing of corn seeds in the culture liquid of micromycetes association, diluted with water in 100– 10,000 times, provides a reliable growth stimulation of seedlings and indicates the absence of phy-totoxicity in microorganisms. The instrumental determination of the content of exogenous phyto-hormones in pre-purified and concentrated phytohormonal extracts shows a significant amount of auxins in a culture fluid — their total amount reaches 18.33 μg/g of dry biomass of the producer, and of cytokinins, in particular, isopentenylidenidine (5.6 μg/g of dry biomass) and zeatin (0.88 μg/g dry biomass). Association T. harzianum 128 in small quantities produces gibberellic acids — GK3 (0.34 μg/g dry biomass) and GK4 — 0.23 μg/g of dry biomass). Absorbent acid was also found in the culture fluid (5.3 μg/g dry biomass), but its amount is four times less than the cor-responding measures in the known strain T. viride F100001, which was used as a positive control in the studies. While the introduction of association T. harzianum 128 into the composted chicken litter substrate, the obtained compost shows high auxin and cytokinin activity. Conclusion. Phyto-hormones, which are produced by the micromycetes association of T. harzianum 128, can positively influence the growth and development of plants, play a protective role in adverse environmental conditions. After introduction of the investigated fungi association to a composted substrate on the basis of chicken litter it accumulates significant amounts of physiologically active substances of auxin and cytokinin action. Under these conditions compost acquires new qualitative features.
The viability and symbiotic properties of active strain Bradyrhizobium japonicum 46 after its long-term storage using different methods were studied. It was established that strain storing in evaporative freezing conditions as well at periodic passages the strain preserves its ability to form active symbiosis with soybean plants. Long-term storage under the layer of mineral oil had negatively influenced on the symbiotic properties of B. japonicum 46 strain resulted in only partial restoration of strains virulence, nitrogen fixing activity and competitiveness.
The effect of organic fertilizers (cattle manure and lupine green manure), as well asmicrobial preparation Biohran on the dynamics of the activity process of nitrogen fixation andN2O emissions in the rhizosphere soil of potato plants, crop yield, and product quality have beeninvestigated. The use of manures stimulates activity of nitrogen fixation, but at the same time,accompanied by a significant loss of gaseous nitrogen compounds. The efficiency of Biohran bythis agrobackground is largely levelled. Lupine green manure stimulates nitrogenase activity,especially in combination with biopreparation. At the same time, there is a tendency to reducenitrous oxide emission. Organic fertilizers contributed to a reliable raise of potato yield. Biohranprovide productivity gains only on the background of green manure. Microbial preparationcontributed to the improvement of quality of production parameters by all studiedagrobackgrounds.
In lysimetric experiment with soybean, the effect of complex inoculants based on Bradyrhizobium japonicum and Azospirillum brasilense on the vertical migration of biogenic elements have been studied. When growing without application of mineral fertilizers, bacterization helped to reduce losses of nitrogen, phosphorus, potassium, calcium, magnesium compounds, as well as moisture and water-soluble humus. Also reduction in loss of biogenic elements compounds under the action of inoculants on the background of N30 was found, but bacterization not affect the loss of moisture and humus. It was shown that complex inoculants affect biometric parameters of soybean plants and enhances the productivity of this culture.
Limitation the number of ixodes ticks is one of the most important tasks of modern science and requires the search for new, highly effective and environmentally hazardous acaricides, so our experiment was aimed at identifying acaricidal properties of essential oils and their individual active components in the population of ixodes ticks in Zaporizhzhia region. The study of acaricidal properties of essential oils was performed in the laboratory conditions. Tampons with wool were soaked in various concentrations of 0.5%, 1%, 3%, 5% and 10% aqueous emulsion of essential oils such as Caryophyllus floris aetheroleum and Limonis aetheroleum, and essential oils of Thymus serpyllum L., Рinus sylvestris L., Salvia officinalis L., Mentha x piperita L. and Eucalyptus viminalis Labill. The essential oils had a significant effect on the lifespan of adult ticks. The results showed the acaricidal effect of Thymus serpyllum essential oils. When searching the acaricidal and repellent properties of individual essential oils components thymole, menthol and citral showed the high activity as acaricides. In the same time, eugenol and borneol proved the effectiveness as repellent. Our research schowed that essential oils are promising as alternative methods of controlling the number of Ixodes ticks in areas where there are conditions for infection with tick infections.
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