Background and Aim: Ensuring the genetic potential of ruminants through nutrition studies using medicinal plants and trace element metals is an urgent task. This study aimed to study the effect of Artemisia absinthium L. (Asteraceae) herb plant separately and in combination with cobalt (II) chloride (CoCl2) chelate compounds on the course of metabolic processes in the rumen, methane concentration, and biochemical blood parameters in bulls.
Materials and Methods: Control group (BD: Basal diet); experimental Group I – BD + A. absinthium herb at a dose of 2.0 g/kg dry matter (DM), experimental Group II – BD + A. absinthium herb at a dose of 2.0 g/kg DM + CoCl2 (1.5 mg/kg), and experimental Group III – BD + CoCl2 were set (1.5 mg/kg). The study was conducted on 16 beef bulls (Kazakh white-headed breed) aged 13–14 months, with an average live weight of 330–335 kg. Enzymatic processes in the rumen were studied, including the level of volatile fatty acids (using the gas chromatography method), nitrogen and its fractions (using the Kjeldahl method), methane concentration, and morphological and biochemical blood composition.
Results: There was a decrease in the concentration of acetic acid in experimental group I (15.9%) (p < 0.05) and in the III group (60.3%) and propionic acid in all experimental groups by 5.6%–47.3% (p < 0.05). Feeding A. absinthium herb as part of the diet of experimental Group I contributed to a decrease in methane concentration by 17.8% (p = 0.05) and the lowest methane concentration was noted for experimental Group III. It was less than in control by 59.1% (p < 0.05). An increase in the concentration of glucose, total protein, and creatinine was found in the experimental groups (p < 0.05). The digestibility of organic matter (3.5%), crude fiber (3.6%), and hemicellulose (11.0%) increased with the feeding of A. absinthium herb.
Conclusion: Thus, using biocomplexes based on A. absinthium herb and CoCl2 do not harm the rumen fermentation of cattle. Still, further microbiome studies are required to evaluate the effects of A. absinthium on cattle properly.
The paper considers the biological effect of probiotic, enzyme and antibiotic agents on metabolism, efficiency and the element status of chickens. Joint inclusion of soybean-bifidum (0.7 ml/kg of feed), Tselloviridin G20x and Biovit-80 resulted in the increase of the egg weight by 9.8 and 9.0%, respectively in comparison with other groups. The group receiving Sporobacterin (10 ml/kg of feed) with feed was characterized by high fat availability (68%). The greatest conversion of feed gross energy was observed in groups in case of joint inclusion of Tselloviridin with probiotic and antibiotic agents (2.8 and 3.0%) and crude protein (6.2 and 7.0%). The spectral microelement analysis showed that the additional inclusion of biologically active agents caused their redistribution towards the increase of the need for main micronutrients for metabolism. All groups were characterized by a reliable increase of essential and conditional-essential elements such as Ca, K, Mg, Na, P, Cr, Cu, Fe, I, With, Mn, Se, Zn against the background of decrease such toxic elements as As, Al, Ag, Sr, Pb, Cd. The received results confirm the modeling role of probiotics, enzymes and antibiotics in the exchange of micro- and macronutrients.
Article presents results of study on effect of various vegetable fats on digestibility of the main nutrients in young cattle. We obtained new data on effects of fats on absorption of amino acids, analyzed blood of experimental animals, depending on source of lipids. We found that use of native form of fat contributes to less intensive digestion of raw fat, in contrast to prepared. In this case, fatty acid composition plays significant role. Transformation of amino acids showed slight difference between the samples. As result of our research, thanks to unique surgical operation on transplantation of the pancreatic duct into isolated segment of intestine, new knowledge was obtained about exocrine function of pancreas, absorption of nutrients, and biochemical parameters of blood in cattle when exposed to fats in animals. Thus, obtained the data reflect body’s response to various types of fats, administered in doses that can be digested by body, without disrupting metabolism of other substances.
The degree of the dry matter (DM) digestibility of the diet and the effect of additional administration of linseed oil on the composition of the rumen microbiome of cattle were studied. The in vitro method was used using the incubator "ANKOM Daisy II "(modifications D200 and D200I) according to a specialized technique. Microflora analysis was performed using MiSeq ("Illumina", USA) by the new generation sequencing method (NGS) with a set of MiSeq reagents. With the introduction of linseed oil, an increase in the digestibility of DM feed by 2.3% relative to the control (wheat bran) was noted. In the experimental group the level of species diversity was 45% higher than in the control group. The Simson dominance index in the control group was 0.44, in the experimental group it is 0.54. The Shannon index was equal to E=0.3, which shows a greater alignment of the microbial community in the ruminal fluid when flaxseed oil was included. There was an increase in the number of bacteria of the phylum Bacteroidetes (64.2 %) and a decrease in Firmicutes (19.3 %)., There was an increase in the number of microorganisms belonging to the classes Saccharibacteria, Spirochaetia, Mollicutes, Lentisphaeria, Elusimicrobia in the experimental group, relative to the control. Also it is an increase in representatives of all classes of the phylum Proteobacteria in comparison with the control by 44 % (p < 0.05).
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.