ZusammenfassungDas DEGEA-Curriculum, basierend auf der S3-Leitlinie „Sedierung in der gastrointestinalen Endoskopie, bietet einen qualitätsorientierten Rahmen, um Endoskopiefachpersonal in Sedierung und Notfallmanagement zu schulen. Dieses Curriculum wurde nun aktualisiert.
The practicability of quality assurance in immunohistochemistry and its integration into the diagnostic process were both tested in this Germany-wide interlaboratory trial. One hundred seventy-two pathologists received one hematoxylin and eosin and five unstained slides from five cases; all cases were selected by a panel because immunohistochemistry was required for their final diagnosis. Participants rendered a morphologic diagnosis and then substantiated it immunohistochemically. Stained slides and evaluation sheets were reviewed by the panel, and the diagnostic process was analyzed in individual steps: morphologic diagnosis, selection of antibodies, staining quality, interpretation of stained slides, conclusions, and final diagnosis. Diagnosis-independent immunohistochemical performance was tested using a multisample tissue block (30 samples) that was stained and evaluated for six common antigens. For individual cases, corresponding to their difficulty, 21-89% of the final diagnoses (altogether 57% from 828 diagnoses) were correct. In a statistical analysis, the tentative diagnosis, the interpretation of stains and conclusions drawn from immunohistochemistry, were independent factors in reaching the diagnosis. Sensitivity to detect estrogen receptors on the multisample tissue block was only 48%. However, 24% of the stains were interpreted as falsely negative. The low staining sensitivity was not correlated to the number of correct diagnoses. The major problem of applying immunohistochemistry in surgical pathology appears to be its integration into the diagnostic process and not the staining quality. Both future quality control projects and training will have to regard these integrative requirements. Multisample tissue blocks provide a promising tool to standardize quantitative immunohistochemical parameters, such as receptor or proliferation scores.
Background: The delegation of medical tasks to trained nurses is still little discussed in Germany. Methods: To get a picture of the current extent of non-physician endoscopy worldwide, we performed a systematic literature research. The following databases were used: CINAHL®, Cochrane Library, the German National Library of Medicine (ZB MED), OPAC, DIMDI, PubMed and MEDLINE. The research words were: nurse and practitioners or nurse clinician and diagnosis and organization and administration. Actual journals and references were used to find relevant studies (hand research). Results: Non-physician endoscopy has been well established in some European countries, in the USA and in several other countries in the western world. Conclusion: The implementation of such programs should be based on solid scientific consideration and evaluated within the framework of the ‘delegation rights'.
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