Thermophiles are the microorganisms which thrive under extreme conditions such as high temperature, making them significant for scientific interest. This study provides information based on isolation of thermophilic strain from Surajkund and Ramkund, hot spring of Jharkhand at 50˚C, 60˚C and 70˚C. Two of the best isolates were used for the extraction of exopolysaccharides. Additionally, the lyophilized product obtained was further analyzed for protein and total sugar estimation. The FTIR analysis revealed the presence of different functional groups such as hydroxyl, C-H stretching, vibration of aliphatic CH 2 and glycosidic linkage, thus proving the product obtained from bacteria was an exopolysaccharides The FESEM analysis of exopolysaccharides show varying surface morphology that is from porous to globular structure. Based on 16S rRNA sequences, the isolates from Surajkund (ON795919) and Ramkund (ON795916) were different strains of Bacillus licheniformis. This is the first report on exopolysaccharide secreting thermophilic strain from these hot springs.
Hot springs serve as a hub for microbial diversity, the microflora of the hot springs can be a source of numerous biologically important molecules. Extremophile bacteria were isolated from different hot springs of India to investigate their potential in industrial applications. The analysis involved both culture dependent and culture independent methods. The metagenomic analysis of the microflora was carried out using next-gen sequencing. Also, thermophilic isolates were culture and primarily screened for amylase, xylanase and cellulase enzyme production. The production for amylase was optimized and partial purification was carried out. Partially purified amylase was tested and characterized using; UPLC, DLS-ZP and TGA. The retention time for the amylase was observed to be around 0.5 minutes along with a stable thermo gravimetric profile around 400oC confirming its stability at higher temperatures. One of the thermophilic isolate was able to degrade lignocellulosic waste exhibiting the potential of isolates from the kund.
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